Determination of azatadine in human plasma by liquid chromatography/tandem mass spectrometry
A sensitive method using liquid chromatography with tandem mass spectrometric detection (LC–MS/MS) was developed and validated for the analysis of antihistamine drug azatadine in human plasma. Loratadine was used as internal standard (IS). Analytes were extracted from human plasma by liquid/liquid e...
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Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2011-08, Vol.879 (23), p.2189-2193 |
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container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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creator | Zhu, Yan-rong Jia, Yan-yan Jiang, Ling Wang, Chao Ding, Li-kun Yang, Jing Li, Liang Zhao, Pei-xi Liu, Wen-xin Yi-Ding Wang, Li Wen, Ai-dong |
description | A sensitive method using liquid chromatography with tandem mass spectrometric detection (LC–MS/MS) was developed and validated for the analysis of antihistamine drug azatadine in human plasma. Loratadine was used as internal standard (IS). Analytes were extracted from human plasma by liquid/liquid extraction using ethyl acetate. The organic phase was reduced to dryness under a stream of nitrogen at 30
°C and the residue was reconstituted with the mobile phase. 5
μL of the resulting solution was injected onto the LC–MS/MS system. A 4.6
mm
×
150
mm, I.D. 5
μm, Agilent TC-C
18 column was used to perform the chromatographic analysis. The mobile phase consisted of ammonium formate buffer 0.010
M (adjusted to pH 4.3 with 1
M formic acid)/acetonitrile (20:80,
v/
v) The chromatographic run time was 5
min per injection and flow rate was 0.6
mL/min. The retention time was 2.4 and 4.4
min for azatadine and IS, respectively. The tandem mass spectrometric detection mode was achieved with electrospray ionization (ESI) iron source and the multiple reaction monitoring (MRM) (291.3
→
248.2
m/
z for azatadine, 383.3
→
337.3
m/
z for IS) was operated in positive ion modes. The low limit of quantitation (LLOQ) was 0.05
ng/mL. The intra-day and inter-day precision of the quality control (QC) samples was 8.93–11.57% relative standard deviation (RSD). The inter-day accuracy of the QC samples was 96.83–105.07% of the nominal values. |
doi_str_mv | 10.1016/j.jchromb.2011.05.050 |
format | Article |
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°C and the residue was reconstituted with the mobile phase. 5
μL of the resulting solution was injected onto the LC–MS/MS system. A 4.6
mm
×
150
mm, I.D. 5
μm, Agilent TC-C
18 column was used to perform the chromatographic analysis. The mobile phase consisted of ammonium formate buffer 0.010
M (adjusted to pH 4.3 with 1
M formic acid)/acetonitrile (20:80,
v/
v) The chromatographic run time was 5
min per injection and flow rate was 0.6
mL/min. The retention time was 2.4 and 4.4
min for azatadine and IS, respectively. The tandem mass spectrometric detection mode was achieved with electrospray ionization (ESI) iron source and the multiple reaction monitoring (MRM) (291.3
→
248.2
m/
z for azatadine, 383.3
→
337.3
m/
z for IS) was operated in positive ion modes. The low limit of quantitation (LLOQ) was 0.05
ng/mL. The intra-day and inter-day precision of the quality control (QC) samples was 8.93–11.57% relative standard deviation (RSD). The inter-day accuracy of the QC samples was 96.83–105.07% of the nominal values.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2011.05.050</identifier><identifier>PMID: 21737359</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; antihistamines ; Azatadine ; Biological and medical sciences ; Chromatography, Liquid - methods ; Cyproheptadine - analogs & derivatives ; Cyproheptadine - blood ; ethyl acetate ; formic acid ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Histamine Antagonists - blood ; Human plasma ; Humans ; ionization ; LC–MS/MS ; liquid chromatography ; Medical sciences ; monitoring ; nitrogen ; Pharmacokinetics ; Pharmacology. Drug treatments ; quality control ; Spectrometry, Mass, Electrospray Ionization - methods ; streams ; tandem mass spectrometry ; Tandem Mass Spectrometry - methods</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2011-08, Vol.879 (23), p.2189-2193</ispartof><rights>2011 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-80615e0c7e7377897fcaf14584bb2987ed784629f5de03ad3736450a56b35ea73</citedby><cites>FETCH-LOGICAL-c418t-80615e0c7e7377897fcaf14584bb2987ed784629f5de03ad3736450a56b35ea73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jchromb.2011.05.050$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24383581$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21737359$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Yan-rong</creatorcontrib><creatorcontrib>Jia, Yan-yan</creatorcontrib><creatorcontrib>Jiang, Ling</creatorcontrib><creatorcontrib>Wang, Chao</creatorcontrib><creatorcontrib>Ding, Li-kun</creatorcontrib><creatorcontrib>Yang, Jing</creatorcontrib><creatorcontrib>Li, Liang</creatorcontrib><creatorcontrib>Zhao, Pei-xi</creatorcontrib><creatorcontrib>Liu, Wen-xin</creatorcontrib><creatorcontrib>Yi-Ding</creatorcontrib><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Wen, Ai-dong</creatorcontrib><title>Determination of azatadine in human plasma by liquid chromatography/tandem mass spectrometry</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>A sensitive method using liquid chromatography with tandem mass spectrometric detection (LC–MS/MS) was developed and validated for the analysis of antihistamine drug azatadine in human plasma. Loratadine was used as internal standard (IS). Analytes were extracted from human plasma by liquid/liquid extraction using ethyl acetate. The organic phase was reduced to dryness under a stream of nitrogen at 30
°C and the residue was reconstituted with the mobile phase. 5
μL of the resulting solution was injected onto the LC–MS/MS system. A 4.6
mm
×
150
mm, I.D. 5
μm, Agilent TC-C
18 column was used to perform the chromatographic analysis. The mobile phase consisted of ammonium formate buffer 0.010
M (adjusted to pH 4.3 with 1
M formic acid)/acetonitrile (20:80,
v/
v) The chromatographic run time was 5
min per injection and flow rate was 0.6
mL/min. The retention time was 2.4 and 4.4
min for azatadine and IS, respectively. The tandem mass spectrometric detection mode was achieved with electrospray ionization (ESI) iron source and the multiple reaction monitoring (MRM) (291.3
→
248.2
m/
z for azatadine, 383.3
→
337.3
m/
z for IS) was operated in positive ion modes. The low limit of quantitation (LLOQ) was 0.05
ng/mL. The intra-day and inter-day precision of the quality control (QC) samples was 8.93–11.57% relative standard deviation (RSD). The inter-day accuracy of the QC samples was 96.83–105.07% of the nominal values.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>antihistamines</subject><subject>Azatadine</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Liquid - methods</subject><subject>Cyproheptadine - analogs & derivatives</subject><subject>Cyproheptadine - blood</subject><subject>ethyl acetate</subject><subject>formic acid</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Histamine Antagonists - blood</subject><subject>Human plasma</subject><subject>Humans</subject><subject>ionization</subject><subject>LC–MS/MS</subject><subject>liquid chromatography</subject><subject>Medical sciences</subject><subject>monitoring</subject><subject>nitrogen</subject><subject>Pharmacokinetics</subject><subject>Pharmacology. Drug treatments</subject><subject>quality control</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>streams</subject><subject>tandem mass spectrometry</subject><subject>Tandem Mass Spectrometry - methods</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2L1TAUhoMozof-BDUbcdU7J03TpCsZRkeFARc64EIIp-np3FyatpO0wvXXm_FedSkcSCDPyfvyMPZCwEaAqC92m53bxim0mxKE2IDKA4_YqTBaFlLX3x7nu9JQQCnLE3aW0g5AaNDyKTsphZZaquaUfX9HC8XgR1z8NPKp5_gTF-z8SNyPfLsGHPk8YArI2z0f_P3qO_47GZfpLuK83V8sOHYUeMCUeJrJLfmVlrh_xp70OCR6fjzP2e31-69XH4ubzx8-XV3eFK4SZikM1EIROE25lTaN7h32olKmatuyMZo6baq6bHrVEUjscvW6UoCqbqUi1PKcvTn8O8fpfqW02OCTo2HAkaY1WaOb7Kypy0yqA-nilFKk3s7RB4x7K8A-eLU7e_RqH7xaUHkg7708JqxtoO7v1h-RGXh9BDA5HPqIo_PpH1dJI5URmXt14HqcLN7FzNx-yUk1ANSqUioTbw8EZWM_PEWbnKfRUedjVmu7yf-n7C91Y6Ld</recordid><startdate>20110801</startdate><enddate>20110801</enddate><creator>Zhu, Yan-rong</creator><creator>Jia, Yan-yan</creator><creator>Jiang, Ling</creator><creator>Wang, Chao</creator><creator>Ding, Li-kun</creator><creator>Yang, Jing</creator><creator>Li, Liang</creator><creator>Zhao, Pei-xi</creator><creator>Liu, Wen-xin</creator><creator>Yi-Ding</creator><creator>Wang, Li</creator><creator>Wen, Ai-dong</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110801</creationdate><title>Determination of azatadine in human plasma by liquid chromatography/tandem mass spectrometry</title><author>Zhu, Yan-rong ; Jia, Yan-yan ; Jiang, Ling ; Wang, Chao ; Ding, Li-kun ; Yang, Jing ; Li, Liang ; Zhao, Pei-xi ; Liu, Wen-xin ; Yi-Ding ; Wang, Li ; Wen, Ai-dong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-80615e0c7e7377897fcaf14584bb2987ed784629f5de03ad3736450a56b35ea73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>antihistamines</topic><topic>Azatadine</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Liquid - methods</topic><topic>Cyproheptadine - analogs & derivatives</topic><topic>Cyproheptadine - blood</topic><topic>ethyl acetate</topic><topic>formic acid</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Histamine Antagonists - blood</topic><topic>Human plasma</topic><topic>Humans</topic><topic>ionization</topic><topic>LC–MS/MS</topic><topic>liquid chromatography</topic><topic>Medical sciences</topic><topic>monitoring</topic><topic>nitrogen</topic><topic>Pharmacokinetics</topic><topic>Pharmacology. Drug treatments</topic><topic>quality control</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>streams</topic><topic>tandem mass spectrometry</topic><topic>Tandem Mass Spectrometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Yan-rong</creatorcontrib><creatorcontrib>Jia, Yan-yan</creatorcontrib><creatorcontrib>Jiang, Ling</creatorcontrib><creatorcontrib>Wang, Chao</creatorcontrib><creatorcontrib>Ding, Li-kun</creatorcontrib><creatorcontrib>Yang, Jing</creatorcontrib><creatorcontrib>Li, Liang</creatorcontrib><creatorcontrib>Zhao, Pei-xi</creatorcontrib><creatorcontrib>Liu, Wen-xin</creatorcontrib><creatorcontrib>Yi-Ding</creatorcontrib><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Wen, Ai-dong</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Yan-rong</au><au>Jia, Yan-yan</au><au>Jiang, Ling</au><au>Wang, Chao</au><au>Ding, Li-kun</au><au>Yang, Jing</au><au>Li, Liang</au><au>Zhao, Pei-xi</au><au>Liu, Wen-xin</au><au>Yi-Ding</au><au>Wang, Li</au><au>Wen, Ai-dong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of azatadine in human plasma by liquid chromatography/tandem mass spectrometry</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2011-08-01</date><risdate>2011</risdate><volume>879</volume><issue>23</issue><spage>2189</spage><epage>2193</epage><pages>2189-2193</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>A sensitive method using liquid chromatography with tandem mass spectrometric detection (LC–MS/MS) was developed and validated for the analysis of antihistamine drug azatadine in human plasma. Loratadine was used as internal standard (IS). Analytes were extracted from human plasma by liquid/liquid extraction using ethyl acetate. The organic phase was reduced to dryness under a stream of nitrogen at 30
°C and the residue was reconstituted with the mobile phase. 5
μL of the resulting solution was injected onto the LC–MS/MS system. A 4.6
mm
×
150
mm, I.D. 5
μm, Agilent TC-C
18 column was used to perform the chromatographic analysis. The mobile phase consisted of ammonium formate buffer 0.010
M (adjusted to pH 4.3 with 1
M formic acid)/acetonitrile (20:80,
v/
v) The chromatographic run time was 5
min per injection and flow rate was 0.6
mL/min. The retention time was 2.4 and 4.4
min for azatadine and IS, respectively. The tandem mass spectrometric detection mode was achieved with electrospray ionization (ESI) iron source and the multiple reaction monitoring (MRM) (291.3
→
248.2
m/
z for azatadine, 383.3
→
337.3
m/
z for IS) was operated in positive ion modes. The low limit of quantitation (LLOQ) was 0.05
ng/mL. The intra-day and inter-day precision of the quality control (QC) samples was 8.93–11.57% relative standard deviation (RSD). The inter-day accuracy of the QC samples was 96.83–105.07% of the nominal values.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>21737359</pmid><doi>10.1016/j.jchromb.2011.05.050</doi><tpages>5</tpages></addata></record> |
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source | MEDLINE; Elsevier ScienceDirect Journals |
subjects | Analysis Analytical, structural and metabolic biochemistry antihistamines Azatadine Biological and medical sciences Chromatography, Liquid - methods Cyproheptadine - analogs & derivatives Cyproheptadine - blood ethyl acetate formic acid Fundamental and applied biological sciences. Psychology General pharmacology Histamine Antagonists - blood Human plasma Humans ionization LC–MS/MS liquid chromatography Medical sciences monitoring nitrogen Pharmacokinetics Pharmacology. Drug treatments quality control Spectrometry, Mass, Electrospray Ionization - methods streams tandem mass spectrometry Tandem Mass Spectrometry - methods |
title | Determination of azatadine in human plasma by liquid chromatography/tandem mass spectrometry |
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