Principles for designing fluorescent sensors and reporters
Sensors and reporters are among the most exciting tools used in cell biology. Now, they are increasingly used in developmental biology and medicine because they allow us to spy on events in living cells and organisms, including humans, in real time and with high spatial resolution. Herein, we discus...
Gespeichert in:
Veröffentlicht in: | Nature chemical biology 2011-07, Vol.7 (8), p.480-483 |
---|---|
Hauptverfasser: | , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 483 |
---|---|
container_issue | 8 |
container_start_page | 480 |
container_title | Nature chemical biology |
container_volume | 7 |
creator | Lemke, Edward A Schultz, Carsten |
description | Sensors and reporters are among the most exciting tools used in cell biology. Now, they are increasingly used in developmental biology and medicine because they allow us to spy on events in living cells and organisms, including humans, in real time and with high spatial resolution. Herein, we discuss multiple design options for fluorescent sensors and reporters as well as strategies to improve their properties and increase development. |
doi_str_mv | 10.1038/nchembio.620 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_878278862</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2471259131</sourcerecordid><originalsourceid>FETCH-LOGICAL-c355t-649dffd643103d3a9af2de402b9a7e24d38668ae62d54a08cf5ff91d7e94af603</originalsourceid><addsrcrecordid>eNptkEtLxDAURoMozji6cy3FjRs75tU0cSeDLxjQha5LprkZO7RJTaYL_72ReQji6l64h-9-HITOCZ4SzOSNqz-gWzR-Kig-QGNSFDTnXKjD_V7gETqJcYUxE4LIYzSipBQKSzlGt6-hcXXTtxAz60NmIDZL17hlZtvBB4g1uHUWwUUfYqadyQL0PqwhxFN0ZHUb4Ww7J-j94f5t9pTPXx6fZ3fzvGZFsc4FV8ZaIzhLdQ3TSltqgGO6ULoEyg2TQkgNgpqCayxrW1iriClBcW0FZhN0tcntg_8cIK6rrkm12lY78EOsZClpKaWgibz8Q678EFwqV0nFSyIIKxJ0vYHq4GMMYKs-NJ0OXxXB1Y_Rame0SkYTfrHNHBYdmD28U5iAfAPEdHJLCL9P_w38BsL3gu0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>894716135</pqid></control><display><type>article</type><title>Principles for designing fluorescent sensors and reporters</title><source>MEDLINE</source><source>Springer Journals</source><source>Nature</source><creator>Lemke, Edward A ; Schultz, Carsten</creator><creatorcontrib>Lemke, Edward A ; Schultz, Carsten</creatorcontrib><description>Sensors and reporters are among the most exciting tools used in cell biology. Now, they are increasingly used in developmental biology and medicine because they allow us to spy on events in living cells and organisms, including humans, in real time and with high spatial resolution. Herein, we discuss multiple design options for fluorescent sensors and reporters as well as strategies to improve their properties and increase development.</description><identifier>ISSN: 1552-4450</identifier><identifier>EISSN: 1552-4469</identifier><identifier>DOI: 10.1038/nchembio.620</identifier><identifier>PMID: 21769088</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>631/1647/1888/2249 ; 631/1647/666 ; Biochemical Engineering ; Biochemistry ; Bioorganic Chemistry ; Biosensing Techniques - methods ; Cell Biology ; Cell Physiological Phenomena ; Cellular biology ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; commentary ; Fluorescence ; Humans ; Indicators and Reagents ; Sensors</subject><ispartof>Nature chemical biology, 2011-07, Vol.7 (8), p.480-483</ispartof><rights>Springer Nature America, Inc. 2011</rights><rights>Copyright Nature Publishing Group Aug 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c355t-649dffd643103d3a9af2de402b9a7e24d38668ae62d54a08cf5ff91d7e94af603</citedby><cites>FETCH-LOGICAL-c355t-649dffd643103d3a9af2de402b9a7e24d38668ae62d54a08cf5ff91d7e94af603</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nchembio.620$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nchembio.620$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21769088$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lemke, Edward A</creatorcontrib><creatorcontrib>Schultz, Carsten</creatorcontrib><title>Principles for designing fluorescent sensors and reporters</title><title>Nature chemical biology</title><addtitle>Nat Chem Biol</addtitle><addtitle>Nat Chem Biol</addtitle><description>Sensors and reporters are among the most exciting tools used in cell biology. Now, they are increasingly used in developmental biology and medicine because they allow us to spy on events in living cells and organisms, including humans, in real time and with high spatial resolution. Herein, we discuss multiple design options for fluorescent sensors and reporters as well as strategies to improve their properties and increase development.</description><subject>631/1647/1888/2249</subject><subject>631/1647/666</subject><subject>Biochemical Engineering</subject><subject>Biochemistry</subject><subject>Bioorganic Chemistry</subject><subject>Biosensing Techniques - methods</subject><subject>Cell Biology</subject><subject>Cell Physiological Phenomena</subject><subject>Cellular biology</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>commentary</subject><subject>Fluorescence</subject><subject>Humans</subject><subject>Indicators and Reagents</subject><subject>Sensors</subject><issn>1552-4450</issn><issn>1552-4469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNptkEtLxDAURoMozji6cy3FjRs75tU0cSeDLxjQha5LprkZO7RJTaYL_72ReQji6l64h-9-HITOCZ4SzOSNqz-gWzR-Kig-QGNSFDTnXKjD_V7gETqJcYUxE4LIYzSipBQKSzlGt6-hcXXTtxAz60NmIDZL17hlZtvBB4g1uHUWwUUfYqadyQL0PqwhxFN0ZHUb4Ww7J-j94f5t9pTPXx6fZ3fzvGZFsc4FV8ZaIzhLdQ3TSltqgGO6ULoEyg2TQkgNgpqCayxrW1iriClBcW0FZhN0tcntg_8cIK6rrkm12lY78EOsZClpKaWgibz8Q678EFwqV0nFSyIIKxJ0vYHq4GMMYKs-NJ0OXxXB1Y_Rame0SkYTfrHNHBYdmD28U5iAfAPEdHJLCL9P_w38BsL3gu0</recordid><startdate>20110718</startdate><enddate>20110718</enddate><creator>Lemke, Edward A</creator><creator>Schultz, Carsten</creator><general>Nature Publishing Group US</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20110718</creationdate><title>Principles for designing fluorescent sensors and reporters</title><author>Lemke, Edward A ; Schultz, Carsten</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c355t-649dffd643103d3a9af2de402b9a7e24d38668ae62d54a08cf5ff91d7e94af603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>631/1647/1888/2249</topic><topic>631/1647/666</topic><topic>Biochemical Engineering</topic><topic>Biochemistry</topic><topic>Bioorganic Chemistry</topic><topic>Biosensing Techniques - methods</topic><topic>Cell Biology</topic><topic>Cell Physiological Phenomena</topic><topic>Cellular biology</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry/Food Science</topic><topic>commentary</topic><topic>Fluorescence</topic><topic>Humans</topic><topic>Indicators and Reagents</topic><topic>Sensors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lemke, Edward A</creatorcontrib><creatorcontrib>Schultz, Carsten</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Science Journals</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Nature chemical biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lemke, Edward A</au><au>Schultz, Carsten</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Principles for designing fluorescent sensors and reporters</atitle><jtitle>Nature chemical biology</jtitle><stitle>Nat Chem Biol</stitle><addtitle>Nat Chem Biol</addtitle><date>2011-07-18</date><risdate>2011</risdate><volume>7</volume><issue>8</issue><spage>480</spage><epage>483</epage><pages>480-483</pages><issn>1552-4450</issn><eissn>1552-4469</eissn><abstract>Sensors and reporters are among the most exciting tools used in cell biology. Now, they are increasingly used in developmental biology and medicine because they allow us to spy on events in living cells and organisms, including humans, in real time and with high spatial resolution. Herein, we discuss multiple design options for fluorescent sensors and reporters as well as strategies to improve their properties and increase development.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>21769088</pmid><doi>10.1038/nchembio.620</doi><tpages>4</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1552-4450 |
ispartof | Nature chemical biology, 2011-07, Vol.7 (8), p.480-483 |
issn | 1552-4450 1552-4469 |
language | eng |
recordid | cdi_proquest_miscellaneous_878278862 |
source | MEDLINE; Springer Journals; Nature |
subjects | 631/1647/1888/2249 631/1647/666 Biochemical Engineering Biochemistry Bioorganic Chemistry Biosensing Techniques - methods Cell Biology Cell Physiological Phenomena Cellular biology Chemistry Chemistry and Materials Science Chemistry/Food Science commentary Fluorescence Humans Indicators and Reagents Sensors |
title | Principles for designing fluorescent sensors and reporters |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T18%3A25%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Principles%20for%20designing%20fluorescent%20sensors%20and%20reporters&rft.jtitle=Nature%20chemical%20biology&rft.au=Lemke,%20Edward%20A&rft.date=2011-07-18&rft.volume=7&rft.issue=8&rft.spage=480&rft.epage=483&rft.pages=480-483&rft.issn=1552-4450&rft.eissn=1552-4469&rft_id=info:doi/10.1038/nchembio.620&rft_dat=%3Cproquest_cross%3E2471259131%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=894716135&rft_id=info:pmid/21769088&rfr_iscdi=true |