The role of electrolytes on protein adsorption at a hydrophilic solid–water interface

The adsorption of four proteins, RNase A, HEWL, BSA, and β-Lac, was examined on the surface of a negatively charged SiO 2/TiO 2 optical waveguide. The amount of adsorbed protein followed the salt series Mg 2+ > Li + > Na + > K + and for anions Cl − > Br − > I −. This ordering was attr...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2010, Vol.75 (1), p.100-106
Hauptverfasser:	Wendorf, Janet R., Radke, Clayton J., Blanch, Harvey W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption - drug effects Animals Anions - chemistry Buffers Cations - chemistry Cattle Chickens Electrolytes Electrolytes - chemistry Hydrogen-Ion Concentration - drug effects Hydrophobic and Hydrophilic Interactions Lactoglobulins - metabolism Models, Chemical Muramidase - metabolism Protein adsorption Proteins - metabolism Ribonuclease, Pancreatic - metabolism Salt excipients Serum Albumin, Bovine - metabolism Sodium Chloride - pharmacology Solid–liquid interfaces Solutions Surface Properties - drug effects Water - chemistry
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The adsorption of four proteins, RNase A, HEWL, BSA, and β-Lac, was examined on the surface of a negatively charged SiO 2/TiO 2 optical waveguide. The amount of adsorbed protein followed the salt series Mg 2+ > Li + > Na + > K + and for anions Cl − > Br − > I −. This ordering was attributed to protein adsorption being primarily influenced by changes in hydrophobic interactions between the surface and the protein resulting from differences in water structure arising from the presence of electrolytes.
ISSN:	0927-7765 1873-4367
DOI:	10.1016/j.colsurfb.2009.08.015