Identification of bovine papilloma virus 10 in teat warts of cattle by DNase-SISPA

Papilloma viruses are detected and identified by PCR with consensus primers designed from human papilloma virus sequences. These and other primers could not detect papilloma virus in bovine teat wart samples despite repeated attempts. DNase-SISPA, a metagenomic method for identifying viruses, could...

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Veröffentlicht in:	Veterinary microbiology 2011-01, Vol.147 (3), p.416-419
Hauptverfasser:	Rai, Gaurava K., Saxena, Meeta, Singh, Vidya, Somvanshi, Ramesh, Sharma, Bhaskar
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biological and medical sciences BPV-10 Cattle cattle diseases Cattle Diseases - virology cows disease detection DNA, Viral - genetics DNase-SISPA Fundamental and applied biological sciences. Psychology Human viral diseases Infectious diseases Medical sciences Metagenomics microbial genetics Microbiology molecular sequence data new methods nucleotide sequences papilloma Papilloma virus Papillomatosis Papillomaviridae Papillomaviridae - genetics Papillomavirus Infections - veterinary Papillomavirus Infections - virology pathogen identification polymerase chain reaction Polymerase Chain Reaction - veterinary Sequence Alignment sequence analysis Sequence Homology, Nucleic Acid Teat warts teats Tumors Viral diseases viral diseases of animals and humans Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye Warts - veterinary Warts - virology
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Zusammenfassung:	Papilloma viruses are detected and identified by PCR with consensus primers designed from human papilloma virus sequences. These and other primers could not detect papilloma virus in bovine teat wart samples despite repeated attempts. DNase-SISPA, a metagenomic method for identifying viruses, could identify bovine papilloma virus type 10 in bovine teat warts. The sequence comparison between consensus primers and bovine papilloma virus type 10 sequences revealed many differences between consensus primers and BPV-10 sequences. We suggest, DNase-SISPA may be used as an alternate method for papilloma virus diagnosis, in cases where PCR fails to identify papilloma viruses.
ISSN:	0378-1135 1873-2542
DOI:	10.1016/j.vetmic.2010.07.015