Genotype variation of Trypanosoma cruzi isolates from different Brazilian biomes

Chagas disease is an enzootic disease, in which the flagellate Trypanosoma cruzi infects a large variety of animals. Humans are accidentally infected due to the migration into wild environments. To identify T. cruzi discrete typing units (DTUs), 19 Brazilian isolates from different biomes and hosts...

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Veröffentlicht in:	Experimental parasitology 2011, Vol.127 (1), p.308-312
Hauptverfasser:	Araújo, C.A.C., Waniek, P.J., Xavier, S.C.C., Jansen, A.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Brazil Chagas disease Chagas Disease - parasitology Didelphis - parasitology ecosystems Exons - genetics Fundamental and applied biological sciences. Psychology Genotype hosts Human protozoal diseases Humans Infectious diseases Leontopithecus - parasitology Life cycle. Host-agent relationship. Pathogenesis Mammalia Mammals Medical sciences monkeys nucleotide sequences Parasitic diseases parasitology Polymerase Chain Reaction Primate Diseases - parasitology Primates Protozoa Protozoal diseases ribosomal RNA RNA, Ribosomal - genetics Rodent Diseases - parasitology Rodentia TcI–TcIIa-e (TcI–TcVI) DTUs Triatominae - parasitology Triatomines Trypanosoma cruzi Trypanosoma cruzi - classification Trypanosoma cruzi - genetics Trypanosomiasis Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution
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Beschreibung
Zusammenfassung:	Chagas disease is an enzootic disease, in which the flagellate Trypanosoma cruzi infects a large variety of animals. Humans are accidentally infected due to the migration into wild environments. To identify T. cruzi discrete typing units (DTUs), 19 Brazilian isolates from different biomes and hosts were analyzed by PCR amplification of 24Sα rRNA, 18S rRNA and mini-exon gene sequences. The majority of the isolates was classified as TcIIb (TcII) but subtypes TcIIc (TcIII) and TcIId (TcV) were also identified. In addition, in monkeys TcI was detected.
ISSN:	0014-4894 1090-2449
DOI:	10.1016/j.exppara.2010.07.013