Production of poly(3-hydroxybutyrate- co-3-hydroxyvalerate) in recombinant Corynebacterium glutamicum using propionate as a precursor

Lipopolysaccharides free P[3-hydroxybutyrate (3HB)- co-3-hydroxyvalerate (3HV)] production was achieved using recombinant Corynebacterium glutamicum harboring polyhydroxyalkanoate (PHA) biosynthetic genes from Ralstonia eutropha. Cells grown on glucose with feeding of propionate as a precursor of 3H...

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Veröffentlicht in:	Journal of biotechnology 2011-04, Vol.152 (4), p.144-146
Hauptverfasser:	Matsumoto, Ken’ichiro, Kitagawa, Kei, Jo, Sung-Jin, Song, Yuyang, Taguchi, Seiichi
Format:	Artikel
Sprache:	eng
Schlagworte:	Bioreactors biotechnology Biotechnology - methods Cell Culture Techniques composite polymers Copolymer Corynebacterium glutamicum Corynebacterium glutamicum - metabolism Cupriavidus necator - genetics genes glucose lipopolysaccharides Polyesters - metabolism Polyhydroxyalkanoate polyhydroxyalkanoates Polyhydroxyalkanoates - biosynthesis Polyhydroxyalkanoates - genetics Propionate Propionates - metabolism propionic acid Ralstonia eutropha
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Zusammenfassung:	Lipopolysaccharides free P[3-hydroxybutyrate (3HB)- co-3-hydroxyvalerate (3HV)] production was achieved using recombinant Corynebacterium glutamicum harboring polyhydroxyalkanoate (PHA) biosynthetic genes from Ralstonia eutropha. Cells grown on glucose with feeding of propionate as a precursor of 3HV unit accumulated 8–47 wt% of P(3HB- co-3HV). The 3HV fraction in the copolymer was varied from 0 to 28 mol% depending on the propionate concentrations.
ISSN:	0168-1656 1873-4863
DOI:	10.1016/j.jbiotec.2010.07.031