Total Synthesis of the Analgesic Conotoxin MrVIB through Selenocysteine-Assisted Folding

Secs for your folding problems: Selenocysteine (Sec) residues were used to drive the folding of conotoxin MrVIB, a previously “unfoldable” miniprotein with therapeutic potential (see picture). This simple strategy should generally facilitate the folding of peptides and proteins with multiple disulfi...

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Veröffentlicht in:Angewandte Chemie International Edition 2011-07, Vol.50 (29), p.6527-6529
Hauptverfasser: de Araujo, Aline Dantas, Callaghan, Brid, Nevin, Simon T., Daly, Norelle L., Craik, David J., Moretta, Melissa, Hopping, Gene, Christie, Macdonald J., Adams, David J., Alewood, Paul F.
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container_end_page 6529
container_issue 29
container_start_page 6527
container_title Angewandte Chemie International Edition
container_volume 50
creator de Araujo, Aline Dantas
Callaghan, Brid
Nevin, Simon T.
Daly, Norelle L.
Craik, David J.
Moretta, Melissa
Hopping, Gene
Christie, Macdonald J.
Adams, David J.
Alewood, Paul F.
description Secs for your folding problems: Selenocysteine (Sec) residues were used to drive the folding of conotoxin MrVIB, a previously “unfoldable” miniprotein with therapeutic potential (see picture). This simple strategy should generally facilitate the folding of peptides and proteins with multiple disulfide bonds.
doi_str_mv 10.1002/anie.201101642
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source MEDLINE; Access via Wiley Online Library
subjects Amino Acid Sequence
Analgesics - chemical synthesis
Analgesics - pharmacology
Animals
Conotoxins - chemical synthesis
Conotoxins - pharmacology
disulfides
Ganglia, Spinal - cytology
Ganglia, Spinal - drug effects
Inhibitory Concentration 50
ion channels
Molecular Sequence Data
Neurons - drug effects
peptides
Protein Folding
regioselective folding
selenocysteine
Selenocysteine - chemistry
Xenopus laevis
title Total Synthesis of the Analgesic Conotoxin MrVIB through Selenocysteine-Assisted Folding
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