Time-Resolved FRET Biosensor Based on Amine-Functionalized Lanthanide-Doped NaYF4 Nanocrystals

Time-Resolved FRET Biosensor Based on Amine-Functionalized Lanthanide-Doped NaYF4 Nanocrystals

Background elimination and improved sensitivity were achieved by time‐resolved (TR) detection with a FRET biosensor for traces of biomolecules such as avidin at concentrations down to 4.8 nM. As shown in the picture, UV excitation of biotinylated NaYF4:Ce/Tb nanocrystals triggers energy transfer to...

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Veröffentlicht in:Angewandte Chemie International Edition 2011-07, Vol.50 (28), p.6306-6310
Hauptverfasser: Tu, Datao, Liu, Liqin, Ju, Qiang, Liu, Yongsheng, Zhu, Haomiao, Li, Renfu, Chen, Xueyuan
Format: Artikel
Sprache:eng
Schlagworte:
Amines - chemistry
Avidin - analysis
Biosensing Techniques - methods
biosensors
Fluorescein-5-isothiocyanate - chemistry
Fluorescence Resonance Energy Transfer
Fluorides - chemistry
FRET
lanthanides
Lanthanoid Series Elements - chemistry
luminescence
Metal Nanoparticles - chemistry
Nanocrystals
nanoparticles
Time Factors
Ultraviolet Rays
Yttrium - chemistry
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Zusammenfassung:Background elimination and improved sensitivity were achieved by time‐resolved (TR) detection with a FRET biosensor for traces of biomolecules such as avidin at concentrations down to 4.8 nM. As shown in the picture, UV excitation of biotinylated NaYF4:Ce/Tb nanocrystals triggers energy transfer to fluorescein isothiocyanate (FITC), whose long‐lived emission due to FRET can be distinguished from the short‐lived background from direct excitation.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201100303