Generating high-quality protein binders: a large screening effort pays off

A large-scale, multifaceted screening and validation strategy combining traditional and recombinant antibody technologies yielded a broad spectrum of validated protein binders with high binding affinity and specificity applicable to many fields in proteome research.

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Bibliographische Detailangaben
Veröffentlicht in:	Nature methods 2011-07, Vol.8 (7), p.545-546
1. Verfasser:	Nollau, Peter
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/338 631/1647/664 Animals Antibodies, Monoclonal - immunology Binders Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology DNA microarrays Humans Life Sciences Monoclonal antibodies news-and-views Physiological aspects Protein Array Analysis - methods Protein Binding Proteins Proteins - chemistry Proteins - immunology Proteome Proteomics Reproducibility of Results Scientific method src Homology Domains - immunology
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Beschreibung
Zusammenfassung:	A large-scale, multifaceted screening and validation strategy combining traditional and recombinant antibody technologies yielded a broad spectrum of validated protein binders with high binding affinity and specificity applicable to many fields in proteome research.
ISSN:	1548-7091 1548-7105
DOI:	10.1038/nmeth.1632