Towards a Restriction Proteinase: Construction of a Self-Activating Enzyme

Towards a Restriction Proteinase: Construction of a Self-Activating Enzyme

Trypsin variants with redesigned specificity: EK‐trypsin(ogen), produced by replacement of the trypsin “99‐loop” by the KRRK‐sequence from enteropeptidase (enterokinase), exhibits specificity for DDDDK‐containing substrates and is capable of self‐activation.

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Bibliographische Detailangaben
Veröffentlicht in:Chembiochem : a European journal of chemical biology 2011-07, Vol.12 (10), p.1523-1527
Hauptverfasser: Schöpfel, Michael, Tziridis, Anastasia, Arnold, Ulrich, Stubbs, Milton T.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
autoactivation
Cattle
Enzyme Activation
enzyme design
enzymes
Escherichia coli - genetics
Gene Expression
Models, Molecular
Mutagenesis
protein modifications
proteinases
Trypsin - genetics
Trypsin - metabolism
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Zusammenfassung:Trypsin variants with redesigned specificity: EK‐trypsin(ogen), produced by replacement of the trypsin “99‐loop” by the KRRK‐sequence from enteropeptidase (enterokinase), exhibits specificity for DDDDK‐containing substrates and is capable of self‐activation.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201000787