Actinomycete Nonomuraea sp. isolated from Indonesian soil is a new producer of inulin fructotransferase

An actinomycete that excretes inulin fructotransferase to the culture supernatant was able to produce di-d-fructofuranose 1,2′:2,3′ dianhydride (DFA III) from inulin, with the greatest rate of enzyme activity at 65°C and at a pH of 5.5. Through chemotaxonomic and 16S rRNA gene analysis, this strain...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2011-06, Vol.111 (6), p.671-674
Hauptverfasser:	Pudjiraharti, Sri, Takesue, Nobuchika, Katayama, Taiki, Lisdiyanti, Puspita, Hanafi, Muhamad, Tanaka, Michiko, Sone, Teruo, Asano, Kozo
Format:	Artikel
Sprache:	eng
Schlagworte:	Actinomycetales - classification Actinomycetales - genetics Actinomycetales - isolation & purification Actinomycetales - metabolism Actinomycetes chemotaxonomy DFA III DNA, Bacterial - genetics enzyme activity genes Hexosyltransferases - biosynthesis Indonesia inulin Inulin - metabolism Inulin fructotransferase Molecular Sequence Data Nonomuraea Phylogeny ribosomal RNA RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA soil Soil Microbiology
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Zusammenfassung:	An actinomycete that excretes inulin fructotransferase to the culture supernatant was able to produce di-d-fructofuranose 1,2′:2,3′ dianhydride (DFA III) from inulin, with the greatest rate of enzyme activity at 65°C and at a pH of 5.5. Through chemotaxonomic and 16S rRNA gene analysis, this strain was identified as genus Nonomuraea in the Streptosporangiaceae family. This is the first report of an inulin fructotransferase producer in this family.
ISSN:	1389-1723 1347-4421
DOI:	10.1016/j.jbiosc.2011.02.001