A biochemically structured model for ethanol fermentation by Kluyveromyces marxianus: A batch fermentation and kinetic study

► A biochemically structured model for anaerobic lactose fermentation was developed. ► Experimental data were fitted by the model. ► Results of parameters’ estimation were analyzed in terms of parameters’ correlation. ► True yields of the process were determined. ► Several aspects of the fermentations were explained by the model. Anaerobic batch fermentations of ricotta cheese whey (i.e. containing lactose) were performed under different operating conditions. Ethanol concentrations of ca. 22 g L −1 were found from whey containing ca. 44 g L −1 lactose, which corresponded to up to 95% of the theoretical ethanol yield within 15 h. The experimental data could be explained by means of a simple knowledge-driven biochemically structured model that was built on bioenergetics principles applied to the metabolic pathways through which lactose is converted into major products. Use of the model showed that the observed concentrations of ethanol, lactose, biomass and glycerol during batch fermentation could be described within a ca. 6% deviation, as could the yield coefficients for biomass and ethanol produced on lactose. The model structure confirmed that the thermodynamics considerations on the stoichiometry of the system constrain the metabolic coefficients within a physically meaningful range thereby providing valuable and reliable insight into fermentation processes.