Alterations in striatal oxidative stress level produced by pharmacological manipulation of dopamine as shown by a novel synthetic marker molecule
Oxidative stress (OS) is thought to participate in neurodegenerative diseases such as Parkinson’s disease, but the contribution of dopamine metabolism and auto-oxidation to OS in Parkinson’s and other diseases is not clear. Oxidative stress in rat striatum was measured by microdialysis using a novel...
Gespeichert in:
Veröffentlicht in: | Neuropharmacology 2011-07, Vol.61 (1), p.87-94 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Oxidative stress (OS) is thought to participate in neurodegenerative diseases such as Parkinson’s disease, but the contribution of dopamine metabolism and auto-oxidation to OS in Parkinson’s and other diseases is not clear. Oxidative stress in rat striatum was measured by microdialysis using a novel synthetic compound composed of tyrosine and linoleic acid (LT), and determination of the oxidation products LT-OOH and LT-epoxy by HPLC-MS. Since LT is non-diffusible through the microdialysis membrane, the oxidized products formed in microdialyzate reflect oxidation state in the extracellular compartment. The extracellular oxidative stress (OS
ec) was compared with intracellular oxidative stress (OS
ic) as measured by tissue levels of oxidized and reduced glutathione and 7-ketocholesterol. Reserpinization caused an increase in OS
ic but a reduction in OS
ec. Inhibition of both subtypes of monoamine oxidase (MAO-A and MAO-B) with tranylcypromine caused a reduction in both OS
ic and OS
ec whereas selective inhibition of MAO-A with clorgyline caused a reduction in Os
ic but no change in OS
ec. A high dose (10 mg/kg) of amphetamine caused an increase in OS
ec whereas a smaller dose (4 mg/kg) caused a reduction in OS
ec. Both doses of amphetamine reduced OS
ic. The present findings are consistent with a role of monoamine oxidase as well as dopamine auto-oxidation in production of striatal OS.
► Striatal extra- and intra-cellular oxidative stress (OS
ec, OS
ic) was determined. ► Reserpinisation reduced microdialyzate dopamine, increased OS
ic but reduced OS
ec. ► Tranylcypromine reduced both OS
ec and OS
ic despite increasing microdialyzate dopamine, clorgyline decreased OS
ic. ► Amphetamine reduced OS
ic but increased OS
ec in a 10 mg/kg dose. ► MAO and extracellular dopamine contribute to OS
ec determined by novel technique. |
---|---|
ISSN: | 0028-3908 1873-7064 |
DOI: | 10.1016/j.neuropharm.2011.03.006 |