Investigation of cytotoxic, apoptosis-inducing, genotoxic and protective effects of the flavonoid rutin in HTC hepatic cells

Rutin is a flavonoid with antioxidant, vasodilatory, anti-inflammatory and immune-stimulating activities. To study the toxicity of rutin and its protective effect, this work investigated the cytotoxic, apoptosis-inducing, genotoxic and protective effects of rutin in HTC cells. In the MTT assay, the...

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Veröffentlicht in:Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie 2011-07, Vol.63 (5), p.459-465
Hauptverfasser: Cristina Marcarini, Juliana, Ferreira Tsuboy, Marcela Stefanini, Cabral Luiz, Rodrigo, Regina Ribeiro, Lucia, Beatriz Hoffmann-Campo, Clara, Ségio Mantovani, Mário
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Sprache:eng
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Zusammenfassung:Rutin is a flavonoid with antioxidant, vasodilatory, anti-inflammatory and immune-stimulating activities. To study the toxicity of rutin and its protective effect, this work investigated the cytotoxic, apoptosis-inducing, genotoxic and protective effects of rutin in HTC cells. In the MTT assay, the highest concentration tested (810 μM) showed cytotoxicity after 72 h of treatment, where cell viability and cell proliferation was diminished. None of the concentrations of rutin tested induced apoptosis after 24 h treatment. The highest concentration of rutin after 24 h treatment induced DNA damage, shown in the comet assay, but did have a genotoxic effect in the micronucleus test. Rutin was tested against the pro-carcinogenic agent benzo(a)pyrene, at concentrations of 90, 270 and 810 μM, and was found to reduce induced DNA damage significantly. This protective effect of rutin against a pro-carcinogen, suggests an important biological activity for this compound, which can contribute to human health through the diet.
ISSN:0940-2993
1618-1433
DOI:10.1016/j.etp.2010.03.005