Supplementation with estradiol-17β improves porcine oocyte maturation and subsequent embryo development

Metaphase II oocyte production was significantly increased by treatment with E2 during the first half of the total in vitro maturation (IVM) period, which was further evidenced by an increase in monospermic fertilization, blastocyst formation, or blastomere viability of IVF- or somatic cell nuclear...

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Veröffentlicht in:	Fertility and sterility 2011-06, Vol.95 (8), p.2582-2584
Hauptverfasser:	Kim, Ji-Su, Ph.D, Song, Bong-Seok, Ph.D, Lee, Sang-Rae, D.V.M., Ph.D, Yoon, Seung-Bin, B.Sc, Huh, Jae-Won, Ph.D, Kim, Sun-Uk, Ph.D, Kim, Ekyun, Ph.D, Kim, Sang-Hyun, D.V.M., Ph.D, Choo, Young-Kug, Ph.D, Koo, Deog-Bon, Ph.D, Chang, Kyu-Tae, Ph.D
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences blastocyst Blastocyst - drug effects Blastocyst - physiology CDC2 Protein Kinase - metabolism Cells, Cultured Diseases of the digestive system Embryo Culture Techniques embryo development embryogenesis Embryonic Development - drug effects Estradiol - pharmacology estradiol-17β Female Fertilization in Vitro Gynecology. Andrology. Obstetrics in vitro fertilization Internal Medicine IVF IVM Medical sciences Meiosis - drug effects metaphase Mitogen-Activated Protein Kinase 1 - metabolism Mitogen-Activated Protein Kinase 3 - metabolism Nuclear Transfer Techniques Obstetrics and Gynecology oocytes Oocytes - drug effects Oocytes - physiology Phosphorylation Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) SCNT somatic cells Swine Time Factors viability
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Zusammenfassung:	Metaphase II oocyte production was significantly increased by treatment with E2 during the first half of the total in vitro maturation (IVM) period, which was further evidenced by an increase in monospermic fertilization, blastocyst formation, or blastomere viability of IVF- or somatic cell nuclear transfer–derived embryos. Thus, we concluded that transient E2 supplementation could improve the IVM rate and subsequent developmental competence in pigs.
ISSN:	0015-0282 1556-5653
DOI:	10.1016/j.fertnstert.2011.03.013