Packed Column Supercritical Fluid Chromatography/Mass Spectrometry for High-Throughput Analysis

Packed Column Supercritical Fluid Chromatography/Mass Spectrometry for High-Throughput Analysis

A supercritical fluid chromatograph was interfaced to a mass spectrometer, and the system was evaluated for applications requiring high sample throughput. Experiments presented demonstrate the high-speed separation capability of supercritical fluid chromatography (SFC) and the effectiveness of super...

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Veröffentlicht in:Analytical chemistry (Washington) 1999-07, Vol.71 (13), p.2410-2416
Hauptverfasser: Ventura, Manuel C, Farrell, William P, Aurigemma, Christine M, Greig, Michael J
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Sprache:eng
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Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Other chromatographic methods
Scientific imaging
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container_end_page 2416
container_issue 13
container_start_page 2410
container_title Analytical chemistry (Washington)
container_volume 71
creator Ventura, Manuel C
Farrell, William P
Aurigemma, Christine M
Greig, Michael J
description A supercritical fluid chromatograph was interfaced to a mass spectrometer, and the system was evaluated for applications requiring high sample throughput. Experiments presented demonstrate the high-speed separation capability of supercritical fluid chromatography (SFC) and the effectiveness of supercritical fluid chromatography/mass spectrometry (SFC/MS) for fast, accurate determinations of multicomponent mixtures. A high-throughput liquid chromatography/mass spectrometry (LC/MS) analysis cycle time is reduced 3-fold using our general SFC/MS high-throughput method, resulting in substantial time saving for large numbers of samples. Unknown mixture characterization is improved due to the increased selectivity of SFC/MS compared to LC/MS. This was demonstrated with sample mixtures from a 96-well combinatorial library plate. In this paper, we report a negative mode atmospheric pressure chemical ionization (APCI) method for SFC/MS suitable for most of the components in library production mixtures. Flow injection analysis (FIA) also benefits from this SFC/MS system. A broader range of solvents is amenable to the SFC mobile phase compared with standard LC/MS solvents, and solutes elute more rapidly from the SFC/MS system, reducing sample carryover and cycle time. Finally, our instrumental setup allows for facile conversion between LC/MS and SFC/MS modes of operation.
doi_str_mv 10.1021/ac981372h
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subjects Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Other chromatographic methods
Scientific imaging
title Packed Column Supercritical Fluid Chromatography/Mass Spectrometry for High-Throughput Analysis
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