Experimental Evaluation of Rat Cord Blood as a Source of Stem Cells Stimulating Regeneration of the Bone Tissue

We studied subpopulation structure of rat cord blood and its effects on regeneration of the bone tissue. In cord blood leukocytes, cell fractions with phenotypes CD45⁺/CD90⁻, CD45⁻/CD90⁺, CD34⁺/CD45⁺ were determined. The cells adhered to plastic during culturing and had a fibroblast-like morphology....

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Veröffentlicht in:	Bulletin of experimental biology and medicine 2010-10, Vol.149 (4), p.502-504
Hauptverfasser:	Melnikova, A. V, Khasanov, A. G, Sendik, A. I, Sibiryak, S. V
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biomedical and Life Sciences Biomedicine bone regeneration Bone Regeneration - immunology Bone Regeneration - physiology Cell Adhesion Cell Biology Female Fetal Blood - cytology Fetal Blood - transplantation Internal Medicine Laboratory Medicine Leukocytes - immunology Pathology Pregnancy Rats Stem cells Stem Cells - cytology stem cells of the blood Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
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Zusammenfassung:	We studied subpopulation structure of rat cord blood and its effects on regeneration of the bone tissue. In cord blood leukocytes, cell fractions with phenotypes CD45⁺/CD90⁻, CD45⁻/CD90⁺, CD34⁺/CD45⁺ were determined. The cells adhered to plastic during culturing and had a fibroblast-like morphology. Transplantation of rat cord blood cells into modeled femoral bone defects stimulated regeneration of the bone tissue and led to recovery of its anatomical integrity, which was confirmed by X-ray examination and histological analysis. No complete recovery of the bone structure was observed in controls (without cell implantation).
ISSN:	0007-4888 1573-8221
DOI:	10.1007/s10517-010-0979-z