A reliable method for storage of tailed phages

A universal and effective method for long-term storage of bacteriophages has not yet been described. We show that randomly selected tailed phages could be stored inside the infected cells at −80°C without a major loss of phage and host viability. Our results suggest the suitability of this method as...

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Veröffentlicht in:	Journal of microbiological methods 2011-03, Vol.84 (3), p.486-489
Hauptverfasser:	Golec, Piotr, Dąbrowski, Kamil, Hejnowicz, Monika S., Gozdek, Agnieszka, Łoś, Joanna M., Węgrzyn, Grzegorz, Łobocka, Małgorzata B., Łoś, Marcin
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria - virology Bacterial collections Bacteriophage bacteriophages Bacteriophages - physiology Biological and medical sciences Caudovirales Cryopreservation Cryopreservation - methods Fundamental and applied biological sciences. Psychology Microbiology Phage preservation Phage storage storage Techniques used in virology viability Virology
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Beschreibung
Zusammenfassung:	A universal and effective method for long-term storage of bacteriophages has not yet been described. We show that randomly selected tailed phages could be stored inside the infected cells at −80°C without a major loss of phage and host viability. Our results suggest the suitability of this method as a standard for phage preservation. [Display omitted] ► Bacteria freshly infected with a lytic phage survive freezing and thawing. ► Frozen, phage-infected bacteria can serve as a source of free phages. ► Phage storage in bacteria is a reliable method for phage preservation.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2011.01.007