Efficient chimeric promoters derived from full-length and sub-genomic transcript promoters of Figwort mosaic virus (FMV)

Addition of multiple repeats of the FS3 upstream activation sequence (FS3-UAS, −270 to −60) intra-molecularly to the TATA containing core-domain of the FS3 (−151 to +31) promoter resulted in 2–3-folds enhanced promoter activity. The chimeric promoter, FS3-UAS-3X with maximum activity, showed 3.31 ti...

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Veröffentlicht in:	Journal of biotechnology 2011-03, Vol.152 (1), p.58-62
Hauptverfasser:	Ranjan, Rajiv, Patro, Sunita, Kumari, Sangeeta, Kumar, Deepak, Dey, Nrisingha, Maiti, Indu B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Caulimovirus - genetics Chimeric promoter CLSM Figwort mosaic virus Figwort mosaic virus (FMV) Fundamental and applied biological sciences. Psychology GFP GUS Polymerase Chain Reaction Promoter Regions, Genetic - genetics Real-time PCR
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Beschreibung
Zusammenfassung:	Addition of multiple repeats of the FS3 upstream activation sequence (FS3-UAS, −270 to −60) intra-molecularly to the TATA containing core-domain of the FS3 (−151 to +31) promoter resulted in 2–3-folds enhanced promoter activity. The chimeric promoter, FS3-UAS-3X with maximum activity, showed 3.31 times stronger activity in root vascular tissue compared to FS3 promoter and could be used efficiently in translational research.
ISSN:	0168-1656 1873-4863
DOI:	10.1016/j.jbiotec.2011.01.015