Rapid Detection of a Cocaine-Binding Aptamer Using Biological Nanopores on a Chip

Rapid Detection of a Cocaine-Binding Aptamer Using Biological Nanopores on a Chip

This paper describes a methodology for the rapid and highly selective detection of cocaine using a membrane protein channel combined with a DNA aptamer. The DNA aptamer recognizes the cocaine molecule with high selectivity. We successfully detected a low concentration of cocaine (300 ng/mL, the drug...

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Veröffentlicht in:Journal of the American Chemical Society 2011-06, Vol.133 (22), p.8474-8477
Hauptverfasser: Kawano, Ryuji, Osaki, Toshihisa, Sasaki, Hirotaka, Takinoue, Masahiro, Yoshizawa, Satoko, Takeuchi, Shoji
Format: Artikel
Sprache:eng
Schlagworte:
Aptamers, Nucleotide - chemistry
Aptamers, Nucleotide - metabolism
Biosensing Techniques
Cocaine - analysis
Cocaine - metabolism
Models, Biological
Molecular Structure
Nanopores
Oligonucleotide Array Sequence Analysis - methods
Time Factors
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Beschreibung
Zusammenfassung:This paper describes a methodology for the rapid and highly selective detection of cocaine using a membrane protein channel combined with a DNA aptamer. The DNA aptamer recognizes the cocaine molecule with high selectivity. We successfully detected a low concentration of cocaine (300 ng/mL, the drug test cutoff limit) within 60 s using a biological nanopore embedded in a microchip.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja2026085