Interaction of eNOS polymorphism with MTHFR variants increase the risk of diabetic nephropathy and its progression in type 2 diabetes mellitus patients

The present study has investigated the role of endothelial nitric oxide (eNOS) G894T polymorphism and its interaction with methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C variants on the predisposition to diabetic nephropathy and its progression. Using polymerase chain reaction–restrict...

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Veröffentlicht in:	Molecular and cellular biochemistry 2011-07, Vol.353 (1-2), p.23-34
Hauptverfasser:	Jafari, Yazdan, Rahimi, Zohreh, Vaisi-Raygani, Asad, Rezaei, Mansour
Format:	Artikel
Sprache:	eng
Schlagworte:	Aged Albuminuria - complications Albuminuria - genetics Albuminuria - pathology Biochemistry Biomedical and Life Sciences Cardiology Cholesterol Coronary heart disease Development and progression Diabetes Mellitus, Type 2 - complications Diabetic nephropathies Diabetic Nephropathies - complications Diabetic Nephropathies - genetics Diabetic Nephropathies - pathology Diabetic neuropathy Disease Progression Epistasis, Genetic Female Gene Frequency Genetic aspects Genetic Predisposition to Disease Genotype Genotypes Humans Life Sciences Male Medical Biochemistry Methylenetetrahydrofolate Reductase (NADPH2) - genetics Middle Aged Nitric oxide Nitric Oxide Synthase Type III - genetics Odds Ratio Oncology Patients Polymerase Chain Reaction Polymorphism Polymorphism, Restriction Fragment Length Polymorphism, Single Nucleotide Risk Assessment Risk Factors Type 2 diabetes
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Zusammenfassung:	The present study has investigated the role of endothelial nitric oxide (eNOS) G894T polymorphism and its interaction with methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C variants on the predisposition to diabetic nephropathy and its progression. Using polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) method the eNOS G894T and MTHFR polymorphisms were detected in 72 microalbuminuric, 68 macroalbuminuric, and 72 normoalbuinuric type 2 diabetes mellitus (T2DM) patients from Western Iran. The presence of GT and GT + TT genotypes of eNOS were associated with insignificantly 1.86- and 1.68-fold increased risk of macroalbuminuria, respectively and 1.21- and 1.13-fold increased risk of microalbuminuria, respectively. However, the concomitant presence of eNOST and MTHFR 1298C alleles were significantly increased the risk of macroalbuminuria (6.6-fold, P
ISSN:	0300-8177 1573-4919
DOI:	10.1007/s11010-011-0770-0