Deciphering the Complexity of Sainfoin (Onobrychis viciifolia) Proanthocyanidins by MALDI-TOF Mass Spectrometry with a Judicious Choice of Isotope Patterns and Matrixes
Use of superdihydroxybenzoic acid as the matrix enabled the analysis of highly complex mixtures of proanthocyanidins from sainfoin (Onobrychis viciifolia) by MALDI-TOF mass spectrometry. Proanthocyanidins contained predominantly B-type homopolymers and heteropolymers up to 12-mers (3400 Da). Use of...
Gespeichert in:
| Veröffentlicht in: | Analytical chemistry (Washington) 2011-06, Vol.83 (11), p.4147-4153 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4153 |
|---|---|
| container_issue | 11 |
| container_start_page | 4147 |
| container_title | Analytical chemistry (Washington) |
| container_volume | 83 |
| creator | Stringano, Elisabetta Cramer, Rainer Hayes, Wayne Smith, Celia Gibson, Trevor Mueller-Harvey, Irene |
| description | Use of superdihydroxybenzoic acid as the matrix enabled the analysis of highly complex mixtures of proanthocyanidins from sainfoin (Onobrychis viciifolia) by MALDI-TOF mass spectrometry. Proanthocyanidins contained predominantly B-type homopolymers and heteropolymers up to 12-mers (3400 Da). Use of another matrix, 2,6-dihydroxyacetophenone, revealed the presence of A-type glycosylated dimers. In addition, we report here how a comparison of the isotopic adduct patterns, which resulted from Li and Na salts as MALDI matrix additives, could be used to confirm the presence of A-type linkages in complex proanthocyanidin mixtures. Preliminary evidence suggested the presence of A-type dimers in glycosylated prodelphinidins and in tetrameric procyanidins and prodelphinidins. |
| doi_str_mv | 10.1021/ac2003856 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_869592007</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2382310251</sourcerecordid><originalsourceid>FETCH-LOGICAL-a286t-ee98e5c2ac2854e3f615e8436cb455a41ad6d1bed1773d255df15d0c84ca11ee3</originalsourceid><addsrcrecordid>eNplkdFqFDEUhoModq1e-AISBNFejCaZSSZ7WbZWV7ZsofV6yCRnnJTZZJpktPNGPqYpXbugV4HDx3f-nB-h15R8pITRT0ozQkrJxRO0oJyRQkjJnqIFydOC1YQcoRcx3hBCKaHiOTpitJJSUL5Av89A27GHYN0PnHrAK78bB7izaca-w1fKus5bhz9snW_DrHsb8U-rre38YNUJvgxeudR7PStnjXURtzO-ON2crYvr7Tm-UDHiqxF0Cn4HKcz4l009VvjbZLLFTxGvem813C9bR5_8CPhSpQQhq5Qz2ZCCvYP4Ej3r1BDh1f49Rt_PP1-vvhab7Zf16nRTKCZFKgCWErhm-SSSV1B2-Zcgq1LotuJcVVQZYWgLhtZ1aRjnpqPcEC0rrSgFKI_R-wfvGPztBDE1Oxs1DINykOM2Uiz5Mp-7zuTbf8gbPwWXwzWyLktRM77M0MkDpIOPMUDXjMHuVJgbSpr78prH8jL7Zi-c2h2YR_JvWxl4twdU1GrognLaxgNXMcq4qA6c0vEQ6v-FfwBa4q6M</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>873367259</pqid></control><display><type>article</type><title>Deciphering the Complexity of Sainfoin (Onobrychis viciifolia) Proanthocyanidins by MALDI-TOF Mass Spectrometry with a Judicious Choice of Isotope Patterns and Matrixes</title><source>ACS Publications</source><source>MEDLINE</source><creator>Stringano, Elisabetta ; Cramer, Rainer ; Hayes, Wayne ; Smith, Celia ; Gibson, Trevor ; Mueller-Harvey, Irene</creator><creatorcontrib>Stringano, Elisabetta ; Cramer, Rainer ; Hayes, Wayne ; Smith, Celia ; Gibson, Trevor ; Mueller-Harvey, Irene</creatorcontrib><description>Use of superdihydroxybenzoic acid as the matrix enabled the analysis of highly complex mixtures of proanthocyanidins from sainfoin (Onobrychis viciifolia) by MALDI-TOF mass spectrometry. Proanthocyanidins contained predominantly B-type homopolymers and heteropolymers up to 12-mers (3400 Da). Use of another matrix, 2,6-dihydroxyacetophenone, revealed the presence of A-type glycosylated dimers. In addition, we report here how a comparison of the isotopic adduct patterns, which resulted from Li and Na salts as MALDI matrix additives, could be used to confirm the presence of A-type linkages in complex proanthocyanidin mixtures. Preliminary evidence suggested the presence of A-type dimers in glycosylated prodelphinidins and in tetrameric procyanidins and prodelphinidins.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac2003856</identifier><identifier>PMID: 21488615</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Acetophenones - chemistry ; Analytical chemistry ; Biological and medical sciences ; Chemistry ; Classical genetics, quantitative genetics, hybrids ; Dimerization ; Exact sciences and technology ; Fabaceae - chemistry ; Fundamental and applied biological sciences. Psychology ; Genetics of eukaryotes. Biological and molecular evolution ; Glycosylation ; Herbs ; Isotope Labeling ; Mass spectrometry ; Methods, theories and miscellaneous ; Polymers ; Proanthocyanidins - analysis ; Spectrometric and optical methods ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><ispartof>Analytical chemistry (Washington), 2011-06, Vol.83 (11), p.4147-4153</ispartof><rights>Copyright © 2011 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><rights>Copyright American Chemical Society Jun 1, 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a286t-ee98e5c2ac2854e3f615e8436cb455a41ad6d1bed1773d255df15d0c84ca11ee3</citedby><cites>FETCH-LOGICAL-a286t-ee98e5c2ac2854e3f615e8436cb455a41ad6d1bed1773d255df15d0c84ca11ee3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac2003856$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac2003856$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24212564$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21488615$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stringano, Elisabetta</creatorcontrib><creatorcontrib>Cramer, Rainer</creatorcontrib><creatorcontrib>Hayes, Wayne</creatorcontrib><creatorcontrib>Smith, Celia</creatorcontrib><creatorcontrib>Gibson, Trevor</creatorcontrib><creatorcontrib>Mueller-Harvey, Irene</creatorcontrib><title>Deciphering the Complexity of Sainfoin (Onobrychis viciifolia) Proanthocyanidins by MALDI-TOF Mass Spectrometry with a Judicious Choice of Isotope Patterns and Matrixes</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Use of superdihydroxybenzoic acid as the matrix enabled the analysis of highly complex mixtures of proanthocyanidins from sainfoin (Onobrychis viciifolia) by MALDI-TOF mass spectrometry. Proanthocyanidins contained predominantly B-type homopolymers and heteropolymers up to 12-mers (3400 Da). Use of another matrix, 2,6-dihydroxyacetophenone, revealed the presence of A-type glycosylated dimers. In addition, we report here how a comparison of the isotopic adduct patterns, which resulted from Li and Na salts as MALDI matrix additives, could be used to confirm the presence of A-type linkages in complex proanthocyanidin mixtures. Preliminary evidence suggested the presence of A-type dimers in glycosylated prodelphinidins and in tetrameric procyanidins and prodelphinidins.</description><subject>Acetophenones - chemistry</subject><subject>Analytical chemistry</subject><subject>Biological and medical sciences</subject><subject>Chemistry</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Dimerization</subject><subject>Exact sciences and technology</subject><subject>Fabaceae - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Glycosylation</subject><subject>Herbs</subject><subject>Isotope Labeling</subject><subject>Mass spectrometry</subject><subject>Methods, theories and miscellaneous</subject><subject>Polymers</subject><subject>Proanthocyanidins - analysis</subject><subject>Spectrometric and optical methods</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkdFqFDEUhoModq1e-AISBNFejCaZSSZ7WbZWV7ZsofV6yCRnnJTZZJpktPNGPqYpXbugV4HDx3f-nB-h15R8pITRT0ozQkrJxRO0oJyRQkjJnqIFydOC1YQcoRcx3hBCKaHiOTpitJJSUL5Av89A27GHYN0PnHrAK78bB7izaca-w1fKus5bhz9snW_DrHsb8U-rre38YNUJvgxeudR7PStnjXURtzO-ON2crYvr7Tm-UDHiqxF0Cn4HKcz4l009VvjbZLLFTxGvem813C9bR5_8CPhSpQQhq5Qz2ZCCvYP4Ej3r1BDh1f49Rt_PP1-vvhab7Zf16nRTKCZFKgCWErhm-SSSV1B2-Zcgq1LotuJcVVQZYWgLhtZ1aRjnpqPcEC0rrSgFKI_R-wfvGPztBDE1Oxs1DINykOM2Uiz5Mp-7zuTbf8gbPwWXwzWyLktRM77M0MkDpIOPMUDXjMHuVJgbSpr78prH8jL7Zi-c2h2YR_JvWxl4twdU1GrognLaxgNXMcq4qA6c0vEQ6v-FfwBa4q6M</recordid><startdate>20110601</startdate><enddate>20110601</enddate><creator>Stringano, Elisabetta</creator><creator>Cramer, Rainer</creator><creator>Hayes, Wayne</creator><creator>Smith, Celia</creator><creator>Gibson, Trevor</creator><creator>Mueller-Harvey, Irene</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20110601</creationdate><title>Deciphering the Complexity of Sainfoin (Onobrychis viciifolia) Proanthocyanidins by MALDI-TOF Mass Spectrometry with a Judicious Choice of Isotope Patterns and Matrixes</title><author>Stringano, Elisabetta ; Cramer, Rainer ; Hayes, Wayne ; Smith, Celia ; Gibson, Trevor ; Mueller-Harvey, Irene</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a286t-ee98e5c2ac2854e3f615e8436cb455a41ad6d1bed1773d255df15d0c84ca11ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Acetophenones - chemistry</topic><topic>Analytical chemistry</topic><topic>Biological and medical sciences</topic><topic>Chemistry</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>Dimerization</topic><topic>Exact sciences and technology</topic><topic>Fabaceae - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Glycosylation</topic><topic>Herbs</topic><topic>Isotope Labeling</topic><topic>Mass spectrometry</topic><topic>Methods, theories and miscellaneous</topic><topic>Polymers</topic><topic>Proanthocyanidins - analysis</topic><topic>Spectrometric and optical methods</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stringano, Elisabetta</creatorcontrib><creatorcontrib>Cramer, Rainer</creatorcontrib><creatorcontrib>Hayes, Wayne</creatorcontrib><creatorcontrib>Smith, Celia</creatorcontrib><creatorcontrib>Gibson, Trevor</creatorcontrib><creatorcontrib>Mueller-Harvey, Irene</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stringano, Elisabetta</au><au>Cramer, Rainer</au><au>Hayes, Wayne</au><au>Smith, Celia</au><au>Gibson, Trevor</au><au>Mueller-Harvey, Irene</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Deciphering the Complexity of Sainfoin (Onobrychis viciifolia) Proanthocyanidins by MALDI-TOF Mass Spectrometry with a Judicious Choice of Isotope Patterns and Matrixes</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2011-06-01</date><risdate>2011</risdate><volume>83</volume><issue>11</issue><spage>4147</spage><epage>4153</epage><pages>4147-4153</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>Use of superdihydroxybenzoic acid as the matrix enabled the analysis of highly complex mixtures of proanthocyanidins from sainfoin (Onobrychis viciifolia) by MALDI-TOF mass spectrometry. Proanthocyanidins contained predominantly B-type homopolymers and heteropolymers up to 12-mers (3400 Da). Use of another matrix, 2,6-dihydroxyacetophenone, revealed the presence of A-type glycosylated dimers. In addition, we report here how a comparison of the isotopic adduct patterns, which resulted from Li and Na salts as MALDI matrix additives, could be used to confirm the presence of A-type linkages in complex proanthocyanidin mixtures. Preliminary evidence suggested the presence of A-type dimers in glycosylated prodelphinidins and in tetrameric procyanidins and prodelphinidins.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>21488615</pmid><doi>10.1021/ac2003856</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2700 |
| ispartof | Analytical chemistry (Washington), 2011-06, Vol.83 (11), p.4147-4153 |
| issn | 0003-2700 1520-6882 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_869592007 |
| source | ACS Publications; MEDLINE |
| subjects | Acetophenones - chemistry Analytical chemistry Biological and medical sciences Chemistry Classical genetics, quantitative genetics, hybrids Dimerization Exact sciences and technology Fabaceae - chemistry Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Glycosylation Herbs Isotope Labeling Mass spectrometry Methods, theories and miscellaneous Polymers Proanthocyanidins - analysis Spectrometric and optical methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods |
| title | Deciphering the Complexity of Sainfoin (Onobrychis viciifolia) Proanthocyanidins by MALDI-TOF Mass Spectrometry with a Judicious Choice of Isotope Patterns and Matrixes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T19%3A07%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Deciphering%20the%20Complexity%20of%20Sainfoin%20(Onobrychis%20viciifolia)%20Proanthocyanidins%20by%20MALDI-TOF%20Mass%20Spectrometry%20with%20a%20Judicious%20Choice%20of%20Isotope%20Patterns%20and%20Matrixes&rft.jtitle=Analytical%20chemistry%20(Washington)&rft.au=Stringano,%20Elisabetta&rft.date=2011-06-01&rft.volume=83&rft.issue=11&rft.spage=4147&rft.epage=4153&rft.pages=4147-4153&rft.issn=0003-2700&rft.eissn=1520-6882&rft.coden=ANCHAM&rft_id=info:doi/10.1021/ac2003856&rft_dat=%3Cproquest_cross%3E2382310251%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=873367259&rft_id=info:pmid/21488615&rfr_iscdi=true |