Deciphering the Complexity of Sainfoin (Onobrychis viciifolia) Proanthocyanidins by MALDI-TOF Mass Spectrometry with a Judicious Choice of Isotope Patterns and Matrixes
Use of superdihydroxybenzoic acid as the matrix enabled the analysis of highly complex mixtures of proanthocyanidins from sainfoin (Onobrychis viciifolia) by MALDI-TOF mass spectrometry. Proanthocyanidins contained predominantly B-type homopolymers and heteropolymers up to 12-mers (3400 Da). Use of...
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Veröffentlicht in: | Analytical chemistry (Washington) 2011-06, Vol.83 (11), p.4147-4153 |
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Sprache: | eng |
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Zusammenfassung: | Use of superdihydroxybenzoic acid as the matrix enabled the analysis of highly complex mixtures of proanthocyanidins from sainfoin (Onobrychis viciifolia) by MALDI-TOF mass spectrometry. Proanthocyanidins contained predominantly B-type homopolymers and heteropolymers up to 12-mers (3400 Da). Use of another matrix, 2,6-dihydroxyacetophenone, revealed the presence of A-type glycosylated dimers. In addition, we report here how a comparison of the isotopic adduct patterns, which resulted from Li and Na salts as MALDI matrix additives, could be used to confirm the presence of A-type linkages in complex proanthocyanidin mixtures. Preliminary evidence suggested the presence of A-type dimers in glycosylated prodelphinidins and in tetrameric procyanidins and prodelphinidins. |
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ISSN: | 0003-2700 1520-6882 |
DOI: | 10.1021/ac2003856 |