Screening, purification, and identification of the enzyme producing N-( l-α- l-aspartyl)- l-phenylalanine methyl ester from l-isoasparagine and l-phenylalanine methyl ester

Screening was carried out for microorganisms able to produce N-( l-α- l-aspartyl)- l-phenylalanine methyl ester [APM] from l-isoasparagine and l-phenylalanine methyl ester hydrochloride. Of the 422 strains examined, 44 strains belonging to the family Enterobacteriaceae were found to produce APM. The...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2009-09, Vol.108 (3), p.190-193
Hauptverfasser:	Kira, Ikuo, Asano, Yasuhisa, Yokozeki, Kenzo
Format:	Artikel
Sprache:	eng
Schlagworte:	Asparagine - chemistry ASPARTAME ASPARTAMO Biological and medical sciences Biotechnology Biotechnology - methods Catalysis Citrobacter - enzymology Dipeptidase E Dipeptidases - analysis Dipeptidases - chemistry Dipeptidases - isolation & purification Electrophoresis, Polyacrylamide Gel ENSAYO ENTEROBACTERIACEAE Enterobacteriaceae - enzymology Escherichia coli - enzymology Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration IDENTIFICACION IDENTIFICATION l-isoasparagine l-phenylalanine methyl ester Models, Chemical N-( l-α-aspartyl)- l-phenylalanine-1-methyl ester Phenylalanine - analogs & derivatives Phenylalanine - chemistry PURIFICACION PURIFICATION Recombinant Proteins - chemistry Salmonella typhimurium - enzymology Screening Shigella flexneri - enzymology TESTAGE TESTING
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Beschreibung
Zusammenfassung:	Screening was carried out for microorganisms able to produce N-( l-α- l-aspartyl)- l-phenylalanine methyl ester [APM] from l-isoasparagine and l-phenylalanine methyl ester hydrochloride. Of the 422 strains examined, 44 strains belonging to the family Enterobacteriaceae were found to produce APM. The enzyme catalyzing APM production was purified and identified as dipeptidase E.
ISSN:	1389-1723 1347-4421
DOI:	10.1016/j.jbiosc.2009.03.018