Characterization of Aspergillus oryzae glycoside hydrolase family 43 β-xylosidase expressed in Escherichia coli

This is the first report of glycoside hydrolase family 43 β-xylosidase from Aspergillus oryzae. To characterize this enzyme, the recombinant enzyme was expressed in Escherichia coli. Unlike known β-xylosidases from fungal origins, the enzyme did not show substrate ambiguity and was stable at alkalin...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2010-02, Vol.109 (2), p.115-117
Hauptverfasser:	Suzuki, Satoshi, Fukuoka, Mari, Ookuchi, Hikaru, Sano, Motoaki, Ozeki, Kenji, Nagayoshi, Emi, Takii, Yukio, Matsushita, Mayumi, Tada, Sawaki, Kusumoto, Ken-Ichi, Kashiwagi, Yutaka
Format:	Artikel
Sprache:	eng
Schlagworte:	ASPERGILLUS ORYZAE Aspergillus oryzae - enzymology Biological and medical sciences Biotechnology BRAN Chromatography, Thin Layer disaccharides enzymatic hydrolysis enzyme activity Enzyme Stability Enzymes Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology Fungal Proteins - genetics Fungal Proteins - metabolism GENE EXPRESSION gene transfer genes GENETIC ENGINEERING genetically engineered microorganisms GENIE GENETIQUE GLICOSIDASAS GLYCOSIDASE GLYCOSIDASES glycoside hydrolase Glycoside hydrolase family 43 HEMICELLULOSE HEMICELULOSA http://www.fao.org/aos/agrovoc#c_1056 http://www.fao.org/aos/agrovoc#c_15974 http://www.fao.org/aos/agrovoc#c_26375 http://www.fao.org/aos/agrovoc#c_27527 http://www.fao.org/aos/agrovoc#c_3308 http://www.fao.org/aos/agrovoc#c_3554 http://www.fao.org/aos/agrovoc#c_6475 Hydrogen-Ion Concentration INGENIERIA GENETICA messenger RNA pH effects Phylogeny RECOMBINACION RECOMBINAISON Recombinant enzyme RECOMBINATION SALVADO SON Substrate Specificity Wheat bran xylan xylan 1,4-beta-xylosidase xylobiose Xylosidases - metabolism β-Xylosidase
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Beschreibung
Zusammenfassung:	This is the first report of glycoside hydrolase family 43 β-xylosidase from Aspergillus oryzae. To characterize this enzyme, the recombinant enzyme was expressed in Escherichia coli. Unlike known β-xylosidases from fungal origins, the enzyme did not show substrate ambiguity and was stable at alkaline pH.
ISSN:	1389-1723 1347-4421
DOI:	10.1016/j.jbiosc.2009.07.018