Novel drug designing rationale against Brugia malayi microfilariae using herbal extracts

<正>Objective:To explore the effect of herbal polyphenolics on filariasis in vitro.Methods:Two herbal extracts,methanolic extracts of roots of Vitex negundo Linn.（Nirgundi） and leaves of Aegle marmelos Juss.（Beal） in different concentrations ranging from 40-80 ng/mL were tested for their antifilarial activity either alone or in combination with diethyl carbonate（DEC）（300μg/mL） and/or H2O2（0.5 mM）.Results:Combination of DEC and each extract had significant anti-filarial effect.And fractions of both extracts were not effective as crude herbal extract.Conclusions: Such unique pharmacodynamics reported in this study might provide new drug development stratagem against filariasis.