Ester synthesis reaction with CALB displaying yeast whole cell biocatalyst: Effect of organic solvent and initial water content

The effect of the reaction conditions on the ester synthesis reaction with CALB displaying yeast whole cells was determined. Utilization of hydrophobic organic solvent improved the efficiency of the ester synthesis reaction. Also the initial water content was important for the expression of the este...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2009-11, Vol.108 (5), p.369-371
Hauptverfasser:	Tanino, Takanori, Ohno, Takumi, Ogino, Chiaki, Fukuda, Hideki, Kondo, Akihiko
Format:	Artikel
Sprache:	eng
Schlagworte:	BIOCATALIZADOR BIOCATALYSEUR BIOCATALYSTS Biological and medical sciences BIOSINTESIS BIOSYNTHESE BIOSYNTHESIS Biotechnology Candida antarctica Candida antarctica liase B Catalysis Cell Culture Techniques - methods Cell surface display CONTENIDO DE HUMEDAD enzyme activity Ester synthesis reaction ESTERASAS ESTERASE ESTERASES Esterification Esters Esters - metabolism Fundamental and applied biological sciences. Psychology Fungal Proteins http://www.fao.org/aos/agrovoc#c_2670 http://www.fao.org/aos/agrovoc#c_27460 http://www.fao.org/aos/agrovoc#c_4886 http://www.fao.org/aos/agrovoc#c_6724 http://www.fao.org/aos/agrovoc#c_928 Hydrophobicity Lipase - metabolism MOISTURE CONTENT Organic Chemicals - metabolism Organic solvent SACCHAROMYCES CEREVISIAE Solvents Solvents - metabolism TENEUR EN EAU triacylglycerol lipase Water - metabolism Water content
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Zusammenfassung:	The effect of the reaction conditions on the ester synthesis reaction with CALB displaying yeast whole cells was determined. Utilization of hydrophobic organic solvent improved the efficiency of the ester synthesis reaction. Also the initial water content was important for the expression of the ester synthesis activity of CALB displaying yeast whole cells.
ISSN:	1389-1723 1347-4421
DOI:	10.1016/j.jbiosc.2009.05.006