Green tea polysaccharide-conjugates protect human umbilical vein endothelial cells against impairments triggered by high glucose

Hot-water extracts of low-grade green tea were precipitated with ethanol, deproteinized with trichloroacetic acid, neutralized with NaOH and fractionated by DEAE-cellulose DE-52 column chromatography to yield three (3) of unexplored polysaccharide-conjugate fractions termed gTPC1, gTPC2 and gTPC3. M...

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Veröffentlicht in:	International journal of biological macromolecules 2011-07, Vol.49 (1), p.50-54
Hauptverfasser:	Chen, Xiaoqiang, Wang, Yuefei, Wu, Yalin, Han, Baoyu, Zhu, Yuejin, Tang, Xiaolin, Sun, Qinglei
Format:	Artikel
Sprache:	eng
Schlagworte:	amino acid composition Amino Acid Sequence Cell Survival - drug effects cell viability Chemical Fractionation chromatography Chromatography, Gel Diabetes dose response Dose-Response Relationship, Drug Endothelial Cells - drug effects Endothelial Cells - metabolism ethanol Free Radical Scavengers - metabolism Free Radical Scavengers - pharmacology glucose Glucose - metabolism glycemic control Green tea High glucose human umbilical vein endothelial cells Humans patients Plant Extracts - isolation & purification Plant Extracts - pharmacology Plant Proteins - genetics Plant Proteins - metabolism Polysaccharide-conjugates Polysaccharides - isolation & purification Polysaccharides - metabolism Polysaccharides - pharmacology protective effect proteins reactive oxygen species Reactive Oxygen Species - metabolism ROS scavenging sodium hydroxide Tea - chemistry Tetrazolium Salts Thiazoles trichloroacetic acid Umbilical Veins - cytology Vascular complications zeta potential
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