Green tea polysaccharide-conjugates protect human umbilical vein endothelial cells against impairments triggered by high glucose

Hot-water extracts of low-grade green tea were precipitated with ethanol, deproteinized with trichloroacetic acid, neutralized with NaOH and fractionated by DEAE-cellulose DE-52 column chromatography to yield three (3) of unexplored polysaccharide-conjugate fractions termed gTPC1, gTPC2 and gTPC3. M...

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Veröffentlicht in:	International journal of biological macromolecules 2011-07, Vol.49 (1), p.50-54
Hauptverfasser:	Chen, Xiaoqiang, Wang, Yuefei, Wu, Yalin, Han, Baoyu, Zhu, Yuejin, Tang, Xiaolin, Sun, Qinglei
Format:	Artikel
Sprache:	eng
Schlagworte:	amino acid composition Amino Acid Sequence Cell Survival - drug effects cell viability Chemical Fractionation chromatography Chromatography, Gel Diabetes dose response Dose-Response Relationship, Drug Endothelial Cells - drug effects Endothelial Cells - metabolism ethanol Free Radical Scavengers - metabolism Free Radical Scavengers - pharmacology glucose Glucose - metabolism glycemic control Green tea High glucose human umbilical vein endothelial cells Humans patients Plant Extracts - isolation & purification Plant Extracts - pharmacology Plant Proteins - genetics Plant Proteins - metabolism Polysaccharide-conjugates Polysaccharides - isolation & purification Polysaccharides - metabolism Polysaccharides - pharmacology protective effect proteins reactive oxygen species Reactive Oxygen Species - metabolism ROS scavenging sodium hydroxide Tea - chemistry Tetrazolium Salts Thiazoles trichloroacetic acid Umbilical Veins - cytology Vascular complications zeta potential
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Zusammenfassung:	Hot-water extracts of low-grade green tea were precipitated with ethanol, deproteinized with trichloroacetic acid, neutralized with NaOH and fractionated by DEAE-cellulose DE-52 column chromatography to yield three (3) of unexplored polysaccharide-conjugate fractions termed gTPC1, gTPC2 and gTPC3. Monosaccharide and amino acid composition, contents of total neutral sugars, proteins and moistures, HPGPC distribution and Zeta potentials of gTPC1–3 were investigated. Exposure of human umbilical vein endothelial (HUVE) cells to high glucose (33mM) for 12h significantly decreased cell viability relative to normal glucose control (p
ISSN:	0141-8130 1879-0003
DOI:	10.1016/j.ijbiomac.2011.03.008