Site-Specific Attachment of Proteins onto a 3D DNA Tetrahedron through Backbone-Modified Phosphorothioate DNA

Phosphorothioate‐modified DNA and short bifunctional linkers are used to attach proteins to a DNA tetrahedron. Precise control of the position of and distance between protein modifications is achieved. This technique is simple, can be adapted to other DNA nanostructures, exhibits high yields, and th...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Small (Weinheim an der Bergstrasse, Germany) Germany), 2011-05, Vol.7 (10), p.1427-1430
Hauptverfasser:	Wong, Ngo Yin, Zhang, Chuan, Tan, Li Huey, Lu, Yi
Format:	Artikel
Sprache:	eng
Schlagworte:	Biotin - analogs & derivatives Biotin - chemistry Biotin - metabolism DNA structures Electrophoretic Mobility Shift Assay Microscopy, Atomic Force nanostructures Nucleic Acid Conformation Organothiophosphorus Compounds - chemistry Organothiophosphorus Compounds - metabolism phosphorothioate Protein Binding proteins Proteins - metabolism self-assembly Streptavidin - metabolism Thionucleotides - chemistry Thionucleotides - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Phosphorothioate‐modified DNA and short bifunctional linkers are used to attach proteins to a DNA tetrahedron. Precise control of the position of and distance between protein modifications is achieved. This technique is simple, can be adapted to other DNA nanostructures, exhibits high yields, and the modification can be programmably placed within the structure.
ISSN:	1613-6810 1613-6829
DOI:	10.1002/smll.201100140