Fast preparation of fungal DNA for PCR screening

Rapid DNA preparation for the quick screening is highly demanded in diverse research fields. Here, we combined an extraction buffer and heat treatment to generate DNA templates from yeast and filamentous fungal materials for PCR. This method may be widely applicable to diverse fungal species in clin...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of microbiological methods 2011-05, Vol.85 (2), p.170-172
Hauptverfasser:	Liu, Kuang-Hung, Yeh, Yu-Ling, Shen, Wei-Chiang
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Chemical Fractionation - methods DNA DNA preparation DNA Primers - genetics DNA, Fungal - genetics DNA, Fungal - isolation & purification Filamentous fungi Fundamental and applied biological sciences. Psychology Fungi - chemistry Fungi - genetics Fungi - isolation & purification heat treatment Humans Microbiology Mycological methods and techniques used in mycology Mycology Mycoses - microbiology PCR screening Polymerase Chain Reaction screening Yeast yeasts
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Rapid DNA preparation for the quick screening is highly demanded in diverse research fields. Here, we combined an extraction buffer and heat treatment to generate DNA templates from yeast and filamentous fungal materials for PCR. This method may be widely applicable to diverse fungal species in clinical and basic studies.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2011.01.029