Injectable Biodegradable Poly(ethylene glycol)/RGD Peptide Hybrid Hydrogels for in vitro Chondrogenesis of Human Mesenchymal Stem Cells

In this study, an injectable and biodegradable poly(ethylene glycol) (PEG)/arginine–glycine–aspartic (RGD) peptide hybrid hydrogel has been synthesized and used as a biomimetic scaffold for encapsulation of human mesenchymal stem cells (hMSCs). Tetrahydroxyl PEG was functionalized with acrylate, and...

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Veröffentlicht in:Macromolecular rapid communications 2010-07, Vol.31 (13), p.1148-1154
Hauptverfasser: Liu, Shao Qiong, Tian, Quan, Wang, Lei, Hedrick, James L., Hui, James Hoi Po, Yang, Yi Yan, Ee, Pui Lai Rachel
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Sprache:eng
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Zusammenfassung:In this study, an injectable and biodegradable poly(ethylene glycol) (PEG)/arginine–glycine–aspartic (RGD) peptide hybrid hydrogel has been synthesized and used as a biomimetic scaffold for encapsulation of human mesenchymal stem cells (hMSCs). Tetrahydroxyl PEG was functionalized with acrylate, and then reacted with thiol‐containing peptide (RGD). Gelation occurred within 30 min with the addition of cells and PEG‐dithiol via Michael addition. The hydrogels synthesized with a peptide concentration of 1.0–5.0 mM achieved significantly greater cell viability when compared to the hydrogels without the RGD peptide. However, the effect of RGD on chondrogenesis was found to be dose‐dependent. Immunohistology studies demonstrated that hMSCs encapsulated in the hydrogel matrix with 1.0 mM RGD and TGF‐β3 showed enhanced positive staining for aggrecan and type II collagen as compared to that with 5.0 mM RGD and unmodified PEG hydrogels. RT‐PCR results further revealed that the cells in hydrogels with 1 mM RGD expressed significantly higher levels of type II collagen than those in PEG hydogels without RGD peptide. These findings have demonstrated that the PEG‐RGD hydrogels can be a promising scaffold to deliver hMSCs for cartilage repair. Injectable and biodegradable PEG/RGD peptide hybrid hydrogels are synthesized via Michael addition to study the effect of RGD content on chondrogenesis of hMSCs. The incorporation of RGD resulted in enhanced cell viability. Importantly, the presence of RGD at an optimal concentration promoted chondrogenesis of hMSCs.
ISSN:1022-1336
1521-3927
DOI:10.1002/marc.200900818