Increase in Ceramide Level after Application of Various Sizes of Sphingomyelin Liposomes to a Cultured Human Skin Model
Sphingomyelin-based liposomes (SPM-L) that were sized (or not) by extrusion through a filter with pores of 100, 200, or 400 nm were applied to a three-dimensional cultured human skin model in order to evaluate which size of SPM-L was most effective at increasing its ceramide level. The diameters of...
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| Veröffentlicht in: | Skin pharmacology and physiology 2011-01, Vol.24 (4), p.218-223 |
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| container_title | Skin pharmacology and physiology |
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| creator | Tokudome, Y. Jinno, M. Todo, H. Kon, T. Sugibayashi, K. Hashimoto, F. |
| description | Sphingomyelin-based liposomes (SPM-L) that were sized (or not) by extrusion through a filter with pores of 100, 200, or 400 nm were applied to a three-dimensional cultured human skin model in order to evaluate which size of SPM-L was most effective at increasing its ceramide level. The diameters of the SPM-L in PBS were 102.7, 181.0, 224.0, and 380.1 nm. The diameters of the liposomes in the culture medium were 117.5, 199.2, 242.1, and 749.8 nm. The diameter of the small liposomes ( |
| doi_str_mv | 10.1159/000324886 |
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The diameters of the SPM-L in PBS were 102.7, 181.0, 224.0, and 380.1 nm. The diameters of the liposomes in the culture medium were 117.5, 199.2, 242.1, and 749.8 nm. The diameter of the small liposomes (<200 nm in diameter) did not change much, at least for 7 days. SPM-L in saline or culture medium were applied to the basal layer side or stratum corneum side of the cultured skin model, and ceramide II, III, V, and VI were then extracted from it. The extracted ceramide molecules were separated by HPTLC, and the concentration of each type of ceramide was quantified using a densitometer. When the small SPM-L (110 or 190 nm in diameter) were applied to the basal layer side, the levels of ceramide III and V were increased. When they were applied to the stratum corneum side, the levels of ceramide II, III, V, and VI were significantly increased compared to those of the PBS group, especially after the application of the small SPM-L (110 nm in diameter). Thus, the application of small SPM-L was useful for increasing the ceramide II, III, V, and VI levels of a cultured human skin model.</description><identifier>ISSN: 1660-5527</identifier><identifier>EISSN: 1660-5535</identifier><identifier>DOI: 10.1159/000324886</identifier><identifier>PMID: 21447992</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>Ceramides - metabolism ; Humans ; Liposomes - administration & dosage ; Original Paper ; Particle Size ; Skin - drug effects ; Skin - metabolism ; Sphingomyelins - administration & dosage ; Tissue Culture Techniques</subject><ispartof>Skin pharmacology and physiology, 2011-01, Vol.24 (4), p.218-223</ispartof><rights>2011 S. Karger AG, Basel</rights><rights>Copyright © 2011 S. Karger AG, Basel.</rights><rights>Copyright (c) 2011 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c477t-771517833a5bb0d48cea18a29d4f7d63db7d7af409818ae1084a67693bbda3e73</citedby><cites>FETCH-LOGICAL-c477t-771517833a5bb0d48cea18a29d4f7d63db7d7af409818ae1084a67693bbda3e73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,2423,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21447992$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tokudome, Y.</creatorcontrib><creatorcontrib>Jinno, M.</creatorcontrib><creatorcontrib>Todo, H.</creatorcontrib><creatorcontrib>Kon, T.</creatorcontrib><creatorcontrib>Sugibayashi, K.</creatorcontrib><creatorcontrib>Hashimoto, F.</creatorcontrib><title>Increase in Ceramide Level after Application of Various Sizes of Sphingomyelin Liposomes to a Cultured Human Skin Model</title><title>Skin pharmacology and physiology</title><addtitle>Skin Pharmacol Physiol</addtitle><description>Sphingomyelin-based liposomes (SPM-L) that were sized (or not) by extrusion through a filter with pores of 100, 200, or 400 nm were applied to a three-dimensional cultured human skin model in order to evaluate which size of SPM-L was most effective at increasing its ceramide level. The diameters of the SPM-L in PBS were 102.7, 181.0, 224.0, and 380.1 nm. The diameters of the liposomes in the culture medium were 117.5, 199.2, 242.1, and 749.8 nm. The diameter of the small liposomes (<200 nm in diameter) did not change much, at least for 7 days. SPM-L in saline or culture medium were applied to the basal layer side or stratum corneum side of the cultured skin model, and ceramide II, III, V, and VI were then extracted from it. The extracted ceramide molecules were separated by HPTLC, and the concentration of each type of ceramide was quantified using a densitometer. When the small SPM-L (110 or 190 nm in diameter) were applied to the basal layer side, the levels of ceramide III and V were increased. When they were applied to the stratum corneum side, the levels of ceramide II, III, V, and VI were significantly increased compared to those of the PBS group, especially after the application of the small SPM-L (110 nm in diameter). Thus, the application of small SPM-L was useful for increasing the ceramide II, III, V, and VI levels of a cultured human skin model.</description><subject>Ceramides - metabolism</subject><subject>Humans</subject><subject>Liposomes - administration & dosage</subject><subject>Original Paper</subject><subject>Particle Size</subject><subject>Skin - drug effects</subject><subject>Skin - metabolism</subject><subject>Sphingomyelins - administration & dosage</subject><subject>Tissue Culture Techniques</subject><issn>1660-5527</issn><issn>1660-5535</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpd0c9v2yAUB3A0rVrTH4fdpwn1Uu2QFQwGfIyiba2Uqoe0vVrP5rmjsY0H9qr0rx9Vshx6Aj0-fPXgEfKZs--c58UVY0xk0hj1gcy4Umye5yL_eNhn-picxPjMWKY0V5_Iccal1EWRzcjLTV8HhIjU9XSJATpnka7wL7YUmhEDXQxD62oYne-pb-gjBOenSNfuFeNbYT38dv2T77bYpoiVG3z0XToaPQW6nNpxCmjp9dRBT9ebRG69xfaMHDXQRjzfr6fk4eeP--X1fHX362a5WM1rqfU415rnXBshIK8qZqWpEbiBrLCy0VYJW2mroZGsMKmMnBkJSqtCVJUFgVqckstd7hD8nwnjWHYu1ti20GN6RmmUllppUSR58U4--yn0qbnSpAaynOUyoW87VAcfY8CmHILrIGxLzsq3WZSHWST7dR84VR3ag_z_-Ql82YENhCcMB7C__w_h84yh</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>Tokudome, Y.</creator><creator>Jinno, M.</creator><creator>Todo, H.</creator><creator>Kon, T.</creator><creator>Sugibayashi, K.</creator><creator>Hashimoto, F.</creator><general>S. 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The diameters of the SPM-L in PBS were 102.7, 181.0, 224.0, and 380.1 nm. The diameters of the liposomes in the culture medium were 117.5, 199.2, 242.1, and 749.8 nm. The diameter of the small liposomes (<200 nm in diameter) did not change much, at least for 7 days. SPM-L in saline or culture medium were applied to the basal layer side or stratum corneum side of the cultured skin model, and ceramide II, III, V, and VI were then extracted from it. The extracted ceramide molecules were separated by HPTLC, and the concentration of each type of ceramide was quantified using a densitometer. When the small SPM-L (110 or 190 nm in diameter) were applied to the basal layer side, the levels of ceramide III and V were increased. When they were applied to the stratum corneum side, the levels of ceramide II, III, V, and VI were significantly increased compared to those of the PBS group, especially after the application of the small SPM-L (110 nm in diameter). Thus, the application of small SPM-L was useful for increasing the ceramide II, III, V, and VI levels of a cultured human skin model.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>21447992</pmid><doi>10.1159/000324886</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Ceramides - metabolism Humans Liposomes - administration & dosage Original Paper Particle Size Skin - drug effects Skin - metabolism Sphingomyelins - administration & dosage Tissue Culture Techniques |
| title | Increase in Ceramide Level after Application of Various Sizes of Sphingomyelin Liposomes to a Cultured Human Skin Model |
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