In vitro development of vitrified buffalo oocytes following parthenogenetic activation and intracytoplasmic sperm injection
The objective of this study was to investigate the potential of swamp buffalo oocytes vitrified-warmed at the metaphase of the second meiotic cell division (M-II) stage to develop to the blastocyst stage after parthenogenetic activation (PA) or intracytoplasmic sperm injection (ICSI). In Experiment...
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| creator | Liang, Y.Y. Phermthai, T. Nagai, T. Somfai, T. Parnpai, R. |
| description | The objective of this study was to investigate the potential of swamp buffalo oocytes vitrified-warmed at the metaphase of the second meiotic cell division (M-II) stage to develop to the blastocyst stage after parthenogenetic activation (PA) or intracytoplasmic sperm injection (ICSI). In Experiment 1, we examined the effects of exposure time of oocytes to cryoprotectants (CPA) on their
in vitro development after PA.
In vitro matured (IVM) oocytes were placed in 10% dimethylsulfoxide (DMSO) + 10% ethylene glycol (EG) for 1 min and then exposed to 20% DMSO + 20% EG + 0.5 M sucrose for 30 s, 45 s or 60 s (1 min + 30 s, 1 min + 45 s and 1 min + 60 s groups, respectively). The oocytes were then exposed to warming solution (TCM199 HEPES + 20% FBS and 0.5M sucrose) for 5 min and then washed in TCM199 HEPES + 20% FBS for 5 min. IVM oocytes without CPA treatments served as a control group. The viability assessed by fluorescein diacetate (FDA) staining was 100% in all groups. The developmental rates after PA to the blastocyst stage between 1min+30s (16%) and control (26%) groups did not differ significantly, but they were significantly higher than those in 1 min + 45 s (10%) and 1 min + 60 s (2%) groups. In Experiment 2, we examined the effect of two CPA exposure times, 1 min + 30 s and 1 min + 45 s on the
in vitro development after PA of oocytes vitrified by the microdrop method. The viabilities in vitrified 1 min + 30 s, 1 min + 45 s and the control (without CPA treatments) groups were not different (97%, 95% and 100%, respectively). The development of surviving oocytes to the blastocyst stage in the vitrified 1 min + 30 s group (8%) was significantly higher than that in the vitrified 1 min + 45 s group (4%) and significantly lower than those in control group (26%). In Experiment 3, we examined the effect of two CPA exposure times, 1 min + 30 s and 1 min + 45 s on
in vitro development after ICSI of vitrified oocytes. Viabilities in vitrified oocytes among 1 min + 30 s, 1 min + 45 s and control groups were not different (96%, 91% and 100%, respectively). After ICSI, vitrified-warmed oocytes were activated and oocytes with the second polar body were cultured for 7 days. The development of ICSI oocytes to the blastocyst stage in the vitrified 1 min + 30 s group (11%) was significantly higher than that in the vitrified 1 min + 45 s (7%) group and significantly lower than those in control group (23%). In conclusion, our study demonstrated that the 1 min + 30 s CPA trea |
| doi_str_mv | 10.1016/j.theriogenology.2010.12.028 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_865690738</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0093691X11000094</els_id><sourcerecordid>865690738</sourcerecordid><originalsourceid>FETCH-LOGICAL-c385t-4c95c03dd1ade2ecff39e0f48ec966a8ef475e912431a4f114b90bbedbede27b3</originalsourceid><addsrcrecordid>eNqNkF1rFDEUhoNU7Fr9CyUXQq9mzcdMdgK9kdJqoeCNgnchk5ysWWaSaZLdsvjnzbi14J1w4MA573s-HoQ-ULKmhIqPu3X5CcnHLYQ4xu1xzcjSYmvC-ldoRfuNbDjj9AytCJG8EZL-OEdvc94RQrgQ9A06Z5R3omvlCv26D_jgS4rYwgHGOE8QCo7uT9E7DxYPe-f0GHGM5lggYxfHMT75sMWzTvWWsJwCxRusTfEHXXwMWAeLfShJV0-cR52n2s8zpKmWd2AW0Tv0ug7O8P45X6Dvd7ffbr40D18_3998emgM77vStEZ2hnBrqbbAwDjHJRDX9mCkELoH1246kJS1nOrWUdoOkgwD2BrANgO_QFenuXOKj3vIRU0-GxhHHSDus-pFJyTZ8L4qr09Kk2LOCZyak590OipK1EJf7dS_9NVCX1GmKv1qv3xetB8msC_mv7ir4O4kgPruwUNS2XgIBqxPlYmy0f_fpt8ZE6To</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>865690738</pqid></control><display><type>article</type><title>In vitro development of vitrified buffalo oocytes following parthenogenetic activation and intracytoplasmic sperm injection</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Liang, Y.Y. ; Phermthai, T. ; Nagai, T. ; Somfai, T. ; Parnpai, R.</creator><creatorcontrib>Liang, Y.Y. ; Phermthai, T. ; Nagai, T. ; Somfai, T. ; Parnpai, R.</creatorcontrib><description>The objective of this study was to investigate the potential of swamp buffalo oocytes vitrified-warmed at the metaphase of the second meiotic cell division (M-II) stage to develop to the blastocyst stage after parthenogenetic activation (PA) or intracytoplasmic sperm injection (ICSI). In Experiment 1, we examined the effects of exposure time of oocytes to cryoprotectants (CPA) on their
in vitro development after PA.
In vitro matured (IVM) oocytes were placed in 10% dimethylsulfoxide (DMSO) + 10% ethylene glycol (EG) for 1 min and then exposed to 20% DMSO + 20% EG + 0.5 M sucrose for 30 s, 45 s or 60 s (1 min + 30 s, 1 min + 45 s and 1 min + 60 s groups, respectively). The oocytes were then exposed to warming solution (TCM199 HEPES + 20% FBS and 0.5M sucrose) for 5 min and then washed in TCM199 HEPES + 20% FBS for 5 min. IVM oocytes without CPA treatments served as a control group. The viability assessed by fluorescein diacetate (FDA) staining was 100% in all groups. The developmental rates after PA to the blastocyst stage between 1min+30s (16%) and control (26%) groups did not differ significantly, but they were significantly higher than those in 1 min + 45 s (10%) and 1 min + 60 s (2%) groups. In Experiment 2, we examined the effect of two CPA exposure times, 1 min + 30 s and 1 min + 45 s on the
in vitro development after PA of oocytes vitrified by the microdrop method. The viabilities in vitrified 1 min + 30 s, 1 min + 45 s and the control (without CPA treatments) groups were not different (97%, 95% and 100%, respectively). The development of surviving oocytes to the blastocyst stage in the vitrified 1 min + 30 s group (8%) was significantly higher than that in the vitrified 1 min + 45 s group (4%) and significantly lower than those in control group (26%). In Experiment 3, we examined the effect of two CPA exposure times, 1 min + 30 s and 1 min + 45 s on
in vitro development after ICSI of vitrified oocytes. Viabilities in vitrified oocytes among 1 min + 30 s, 1 min + 45 s and control groups were not different (96%, 91% and 100%, respectively). After ICSI, vitrified-warmed oocytes were activated and oocytes with the second polar body were cultured for 7 days. The development of ICSI oocytes to the blastocyst stage in the vitrified 1 min + 30 s group (11%) was significantly higher than that in the vitrified 1 min + 45 s (7%) group and significantly lower than those in control group (23%). In conclusion, our study demonstrated that the 1 min + 30 s CPA treatment regimen could yield the highest blastocyst formation rates after PA and ICSI for oocytes vitrified by the microdrop method.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2010.12.028</identifier><identifier>PMID: 21356549</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Blastocyst - cytology ; Blastocyst - drug effects ; Buffalo oocytes ; Buffaloes ; Cryopreservation - veterinary ; Cryoprotective Agents - pharmacology ; Embryo Culture Techniques - veterinary ; Embryonic Development ; Female ; ICSI ; Male ; Microdrop ; Oocytes - drug effects ; Oocytes - growth & development ; Parthenogenesis ; Sperm Injections, Intracytoplasmic - methods ; Sperm Injections, Intracytoplasmic - veterinary ; Vitrification</subject><ispartof>Theriogenology, 2011-06, Vol.75 (9), p.1652-1660</ispartof><rights>2011 Elsevier Inc.</rights><rights>Copyright © 2011 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-4c95c03dd1ade2ecff39e0f48ec966a8ef475e912431a4f114b90bbedbede27b3</citedby><cites>FETCH-LOGICAL-c385t-4c95c03dd1ade2ecff39e0f48ec966a8ef475e912431a4f114b90bbedbede27b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.theriogenology.2010.12.028$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21356549$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liang, Y.Y.</creatorcontrib><creatorcontrib>Phermthai, T.</creatorcontrib><creatorcontrib>Nagai, T.</creatorcontrib><creatorcontrib>Somfai, T.</creatorcontrib><creatorcontrib>Parnpai, R.</creatorcontrib><title>In vitro development of vitrified buffalo oocytes following parthenogenetic activation and intracytoplasmic sperm injection</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description>The objective of this study was to investigate the potential of swamp buffalo oocytes vitrified-warmed at the metaphase of the second meiotic cell division (M-II) stage to develop to the blastocyst stage after parthenogenetic activation (PA) or intracytoplasmic sperm injection (ICSI). In Experiment 1, we examined the effects of exposure time of oocytes to cryoprotectants (CPA) on their
in vitro development after PA.
In vitro matured (IVM) oocytes were placed in 10% dimethylsulfoxide (DMSO) + 10% ethylene glycol (EG) for 1 min and then exposed to 20% DMSO + 20% EG + 0.5 M sucrose for 30 s, 45 s or 60 s (1 min + 30 s, 1 min + 45 s and 1 min + 60 s groups, respectively). The oocytes were then exposed to warming solution (TCM199 HEPES + 20% FBS and 0.5M sucrose) for 5 min and then washed in TCM199 HEPES + 20% FBS for 5 min. IVM oocytes without CPA treatments served as a control group. The viability assessed by fluorescein diacetate (FDA) staining was 100% in all groups. The developmental rates after PA to the blastocyst stage between 1min+30s (16%) and control (26%) groups did not differ significantly, but they were significantly higher than those in 1 min + 45 s (10%) and 1 min + 60 s (2%) groups. In Experiment 2, we examined the effect of two CPA exposure times, 1 min + 30 s and 1 min + 45 s on the
in vitro development after PA of oocytes vitrified by the microdrop method. The viabilities in vitrified 1 min + 30 s, 1 min + 45 s and the control (without CPA treatments) groups were not different (97%, 95% and 100%, respectively). The development of surviving oocytes to the blastocyst stage in the vitrified 1 min + 30 s group (8%) was significantly higher than that in the vitrified 1 min + 45 s group (4%) and significantly lower than those in control group (26%). In Experiment 3, we examined the effect of two CPA exposure times, 1 min + 30 s and 1 min + 45 s on
in vitro development after ICSI of vitrified oocytes. Viabilities in vitrified oocytes among 1 min + 30 s, 1 min + 45 s and control groups were not different (96%, 91% and 100%, respectively). After ICSI, vitrified-warmed oocytes were activated and oocytes with the second polar body were cultured for 7 days. The development of ICSI oocytes to the blastocyst stage in the vitrified 1 min + 30 s group (11%) was significantly higher than that in the vitrified 1 min + 45 s (7%) group and significantly lower than those in control group (23%). In conclusion, our study demonstrated that the 1 min + 30 s CPA treatment regimen could yield the highest blastocyst formation rates after PA and ICSI for oocytes vitrified by the microdrop method.</description><subject>Animals</subject><subject>Blastocyst - cytology</subject><subject>Blastocyst - drug effects</subject><subject>Buffalo oocytes</subject><subject>Buffaloes</subject><subject>Cryopreservation - veterinary</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Embryo Culture Techniques - veterinary</subject><subject>Embryonic Development</subject><subject>Female</subject><subject>ICSI</subject><subject>Male</subject><subject>Microdrop</subject><subject>Oocytes - drug effects</subject><subject>Oocytes - growth & development</subject><subject>Parthenogenesis</subject><subject>Sperm Injections, Intracytoplasmic - methods</subject><subject>Sperm Injections, Intracytoplasmic - veterinary</subject><subject>Vitrification</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkF1rFDEUhoNU7Fr9CyUXQq9mzcdMdgK9kdJqoeCNgnchk5ysWWaSaZLdsvjnzbi14J1w4MA573s-HoQ-ULKmhIqPu3X5CcnHLYQ4xu1xzcjSYmvC-ldoRfuNbDjj9AytCJG8EZL-OEdvc94RQrgQ9A06Z5R3omvlCv26D_jgS4rYwgHGOE8QCo7uT9E7DxYPe-f0GHGM5lggYxfHMT75sMWzTvWWsJwCxRusTfEHXXwMWAeLfShJV0-cR52n2s8zpKmWd2AW0Tv0ug7O8P45X6Dvd7ffbr40D18_3998emgM77vStEZ2hnBrqbbAwDjHJRDX9mCkELoH1246kJS1nOrWUdoOkgwD2BrANgO_QFenuXOKj3vIRU0-GxhHHSDus-pFJyTZ8L4qr09Kk2LOCZyak590OipK1EJf7dS_9NVCX1GmKv1qv3xetB8msC_mv7ir4O4kgPruwUNS2XgIBqxPlYmy0f_fpt8ZE6To</recordid><startdate>20110601</startdate><enddate>20110601</enddate><creator>Liang, Y.Y.</creator><creator>Phermthai, T.</creator><creator>Nagai, T.</creator><creator>Somfai, T.</creator><creator>Parnpai, R.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110601</creationdate><title>In vitro development of vitrified buffalo oocytes following parthenogenetic activation and intracytoplasmic sperm injection</title><author>Liang, Y.Y. ; Phermthai, T. ; Nagai, T. ; Somfai, T. ; Parnpai, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-4c95c03dd1ade2ecff39e0f48ec966a8ef475e912431a4f114b90bbedbede27b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Blastocyst - cytology</topic><topic>Blastocyst - drug effects</topic><topic>Buffalo oocytes</topic><topic>Buffaloes</topic><topic>Cryopreservation - veterinary</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Embryo Culture Techniques - veterinary</topic><topic>Embryonic Development</topic><topic>Female</topic><topic>ICSI</topic><topic>Male</topic><topic>Microdrop</topic><topic>Oocytes - drug effects</topic><topic>Oocytes - growth & development</topic><topic>Parthenogenesis</topic><topic>Sperm Injections, Intracytoplasmic - methods</topic><topic>Sperm Injections, Intracytoplasmic - veterinary</topic><topic>Vitrification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liang, Y.Y.</creatorcontrib><creatorcontrib>Phermthai, T.</creatorcontrib><creatorcontrib>Nagai, T.</creatorcontrib><creatorcontrib>Somfai, T.</creatorcontrib><creatorcontrib>Parnpai, R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liang, Y.Y.</au><au>Phermthai, T.</au><au>Nagai, T.</au><au>Somfai, T.</au><au>Parnpai, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro development of vitrified buffalo oocytes following parthenogenetic activation and intracytoplasmic sperm injection</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2011-06-01</date><risdate>2011</risdate><volume>75</volume><issue>9</issue><spage>1652</spage><epage>1660</epage><pages>1652-1660</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>The objective of this study was to investigate the potential of swamp buffalo oocytes vitrified-warmed at the metaphase of the second meiotic cell division (M-II) stage to develop to the blastocyst stage after parthenogenetic activation (PA) or intracytoplasmic sperm injection (ICSI). In Experiment 1, we examined the effects of exposure time of oocytes to cryoprotectants (CPA) on their
in vitro development after PA.
In vitro matured (IVM) oocytes were placed in 10% dimethylsulfoxide (DMSO) + 10% ethylene glycol (EG) for 1 min and then exposed to 20% DMSO + 20% EG + 0.5 M sucrose for 30 s, 45 s or 60 s (1 min + 30 s, 1 min + 45 s and 1 min + 60 s groups, respectively). The oocytes were then exposed to warming solution (TCM199 HEPES + 20% FBS and 0.5M sucrose) for 5 min and then washed in TCM199 HEPES + 20% FBS for 5 min. IVM oocytes without CPA treatments served as a control group. The viability assessed by fluorescein diacetate (FDA) staining was 100% in all groups. The developmental rates after PA to the blastocyst stage between 1min+30s (16%) and control (26%) groups did not differ significantly, but they were significantly higher than those in 1 min + 45 s (10%) and 1 min + 60 s (2%) groups. In Experiment 2, we examined the effect of two CPA exposure times, 1 min + 30 s and 1 min + 45 s on the
in vitro development after PA of oocytes vitrified by the microdrop method. The viabilities in vitrified 1 min + 30 s, 1 min + 45 s and the control (without CPA treatments) groups were not different (97%, 95% and 100%, respectively). The development of surviving oocytes to the blastocyst stage in the vitrified 1 min + 30 s group (8%) was significantly higher than that in the vitrified 1 min + 45 s group (4%) and significantly lower than those in control group (26%). In Experiment 3, we examined the effect of two CPA exposure times, 1 min + 30 s and 1 min + 45 s on
in vitro development after ICSI of vitrified oocytes. Viabilities in vitrified oocytes among 1 min + 30 s, 1 min + 45 s and control groups were not different (96%, 91% and 100%, respectively). After ICSI, vitrified-warmed oocytes were activated and oocytes with the second polar body were cultured for 7 days. The development of ICSI oocytes to the blastocyst stage in the vitrified 1 min + 30 s group (11%) was significantly higher than that in the vitrified 1 min + 45 s (7%) group and significantly lower than those in control group (23%). In conclusion, our study demonstrated that the 1 min + 30 s CPA treatment regimen could yield the highest blastocyst formation rates after PA and ICSI for oocytes vitrified by the microdrop method.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>21356549</pmid><doi>10.1016/j.theriogenology.2010.12.028</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Blastocyst - cytology Blastocyst - drug effects Buffalo oocytes Buffaloes Cryopreservation - veterinary Cryoprotective Agents - pharmacology Embryo Culture Techniques - veterinary Embryonic Development Female ICSI Male Microdrop Oocytes - drug effects Oocytes - growth & development Parthenogenesis Sperm Injections, Intracytoplasmic - methods Sperm Injections, Intracytoplasmic - veterinary Vitrification |
| title | In vitro development of vitrified buffalo oocytes following parthenogenetic activation and intracytoplasmic sperm injection |
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