On the Relevance of Peptide Sequence Permutations in Shotgun Proteomics Studies

On the Relevance of Peptide Sequence Permutations in Shotgun Proteomics Studies

In collision-induced dissociation (CID) of peptides, it has been observed that rearrangement processes can take place that appear to permute/scramble the original primary structure, which may in principle adversely affect peptide identification. Here, an analysis of sequence permutation in tandem ma...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of proteome research 2011-05, Vol.10 (5), p.2409-2416
Hauptverfasser: Yu, Long, Tan, Yanglan, Tsai, Yihsuan, Goodlett, David R, Polfer, Nick C
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence - genetics
Molecular Sequence Data
Peptides - genetics
Proteomics - methods
Pseudomonas aeruginosa - chemistry
Tandem Mass Spectrometry
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:In collision-induced dissociation (CID) of peptides, it has been observed that rearrangement processes can take place that appear to permute/scramble the original primary structure, which may in principle adversely affect peptide identification. Here, an analysis of sequence permutation in tandem mass spectra is presented for a previously published proteomics study on P. aeruginosa (Scherl et al., J. Am. Soc. Mass Spectrom. 2008, 19, 891) conducted using an LTQ-orbitrap. Overall, 4878 precursor ions are matched by considering the accurate mass (i.e.,
ISSN:1535-3893
1535-3907
DOI:10.1021/pr101235w