Proline induces alterations in nucleotide hydrolysis in rat blood serum

The main objective of the present study was to evaluate the in vivo (acute and chronic) and in vitro effects of proline on serum nucleotide hydrolysis. For acute administration, 29-day-old rats received one subcutaneous injection of proline (18.2 (μmol/g body weight) or an equivalent volume of 0.9% saline solution (control) and were sacrificed 1 h, 3 h or 12 h later. Results showed that acute proline administration provoked a decrease in ATP (42%) and ADP (49%) hydrolysis when rats were sacrificed 1 h after the injection. Furthermore, in rats killed 3 h and 12 h after acute injection, no change in nucleotide hydrolysis were observed. For chronic treatment, buffered proline was injected subcutaneously twice a day at 10 h intervals from the 6th to the 28th day of age. Rats were sacrificed 3 h or 12 h after the last injection. Chronic administration of proline did not alter the nucleotide hydrolysis when the rats were killed 12 h after the last injection, but decreased ATP (15%) and ADP (32%) hydrolysis when rats were sacrificed 3 h after the last injection. The in vitro effect of proline (3.0 μM - 1.0 mM) on serum nucleotide hydrolysis was also investigated; results showed that 1.0 mM of proline significantly increased ATP (45%), ADP (55%) and AMP (49%) hydrolysis. The data indicate that proline in vivo and in vitro alters nucleotide hydrolysis, which may be involved in the pathogeny of hyperprolinemic patients.