A simple PCR/RFLP analysis can differentiate between Candida albicans, Aspergillus niger, and Aspergillus fumigatus

The clinical management of immunocompromised patients depends on the rapid identification of infectious agents such as fungal pathogens. The procedure described here for accomplishing this uses a sensitive polymerase chain reaction method, previously reported, combined with restriction-enzyme digest...

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Veröffentlicht in:	Molecular biotechnology 2003-07, Vol.24 (3), p.229-231
Hauptverfasser:	ISIK, Nilgun, WHITE, Lewis, BARNES, Rosemary, POYNTON, Christopher J, MILLS, Ken I
Format:	Artikel
Sprache:	eng
Schlagworte:	Aspergillus Aspergillus fumigatus Aspergillus fumigatus - classification Aspergillus fumigatus - genetics Aspergillus fumigatus - isolation & purification Aspergillus niger Aspergillus niger - classification Aspergillus niger - genetics Aspergillus niger - isolation & purification Bacteria Base Sequence Biological and medical sciences Candida albicans Candida albicans - classification Candida albicans - genetics Candida albicans - isolation & purification DNA Primers Enzymes Fundamental and applied biological sciences. Psychology Fungi Humans Immunocompromised hosts Pathogens Polymerase chain reaction Polymerase Chain Reaction - methods Polymorphism, Restriction Fragment Length Sensitivity and Specificity Species classification
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Beschreibung
Zusammenfassung:	The clinical management of immunocompromised patients depends on the rapid identification of infectious agents such as fungal pathogens. The procedure described here for accomplishing this uses a sensitive polymerase chain reaction method, previously reported, combined with restriction-enzyme digestion to distinguish between Candida and Aspergillus species and to classify Aspergillus strains.
ISSN:	1073-6085 1559-0305 1073-6085
DOI:	10.1385/MB:24:3:229