Bioelectrical impedance analysis determination of water content and distribution in the horse

A horse’s hydration status is critical to its health. The accurate and quantitative determination of it has been problematic because of size, length and density of hair, and uneven topography. The objective of this study was to validate a bioelectrical impedance analysis (BIA) method for objectively quantifying hydration status. Monofrequency BIA values and simple biometric measurements were used to construct predictive equations for total body water, plasma, extracellular, and intra-cellular fluid volumes. These predictive equations were correlated with standard body fluid dilution reference methods. The result was an accuracy of ±0.64% for total body water, ±0.17% for plasma volume, ±1.91% for extra-cellular fluid, and ±0.57% for intra-cellular fluid compartments. Less than 5 min was required for all of the measurements and determinations. Therefore, it appears that an accurate measurement of body fluid distribution can be performed on horses using a fast, easy, non-invasive, inexpensive BIA method.