A Ferritin-Based Label for Cellular Electron Cryotomography

Electron cryotomography provides nanometer resolution structural detail of thin biological specimens in a near-native state. Currently, its application is limited by the lack of a specific label for the identification of molecules. Our aim was to develop such a label, analogous to GFP used in fluore...

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Veröffentlicht in:Structure (London) 2011-02, Vol.19 (2), p.147-154
Hauptverfasser: Wang, Qing, Mercogliano, Christopher P., Löwe, Jan
Format: Artikel
Sprache:eng
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Zusammenfassung:Electron cryotomography provides nanometer resolution structural detail of thin biological specimens in a near-native state. Currently, its application is limited by the lack of a specific label for the identification of molecules. Our aim was to develop such a label, analogous to GFP used in fluorescence microscopy. Here, we demonstrate the use of Escherichia coli ferritin FtnA protein as a clonable label for electron cryotomography. Overproduced ferritin is visible in E. coli cells using cryotomography and fusing this label to a short membrane targeting sequence correctly directs the ferritin fusion to the membrane. Using two proteins with known subcellular localization patterns with this ferritin tag, also including GFP, we obtained essentially the same labeling patterns using electron cryotomography as compared with fluorescence light microscopy. Hence, the ferritin label localizes efficiently and faithfully and it will be a valuable tool for the unambiguous identification of molecules in cellular electron cryotomograms. ► Iron-loaded FtnA ferritin particles are visible in electron cryotomograms ► A membrane-targeting peptide correctly directs the ferritin label to the membrane ► CheY-ferritin directs the label to the chemotaxis array near the cell poles ► ZapA-ferritin directs the label to the septum where FtsZ is located
ISSN:0969-2126
1878-4186
DOI:10.1016/j.str.2010.12.002