Photochemical Ligation of DNA Probe prepared in Click Chemistry

Photochemical Ligation of DNA Probe prepared in Click Chemistry

Template-directed photoligation with 5-carboxyvinyl-2'-deoxyuridine (CVU) can be used for genomic research, biotechnology and biomedical agents. However, in photoligation with CVU, a long photoirradiation time at 366 nm is required to complete photoligation. To overcome the limitation of photol...

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Veröffentlicht in:Journal of Photopolymer Science and Technology 2009/06/30, Vol.22(2), pp.267-272
Hauptverfasser: Yoshimura, Yoshinaga, Matsuzaki, Tomoya, Fujimoto, Kenzo
Format: Artikel
Sprache:eng
Schlagworte:
click chemistry
DNA
photoligation
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Zusammenfassung:Template-directed photoligation with 5-carboxyvinyl-2'-deoxyuridine (CVU) can be used for genomic research, biotechnology and biomedical agents. However, in photoligation with CVU, a long photoirradiation time at 366 nm is required to complete photoligation. To overcome the limitation of photoligation with CVU, we synthesized new photosensitive probes in the copper-catalyzed azide-alkyne cycloaddition reaction, as the best example of click chemistry.
ISSN:0914-9244
1349-6336
1349-6336
DOI:10.2494/photopolymer.22.267