Rapid DNA detection by interface PCR on nanoparticles

A novel, rapid DNA detection method based on fluorescence quenching of quantum dots (QDs) by gold nanoparticles (GNPs) through polymerase chain reaction (PCR) was developed. In proof-of-concept experiments, the length of the amplicon DNA ranging from 152 to 1003 base pairs (bp) could be determined b...

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Veröffentlicht in:	Biosensors & bioelectronics 2011-01, Vol.26 (5), p.2495-2499
Hauptverfasser:	Kuang, Hua, Zhao, Shuge, Chen, Wei, Ma, Wei, Yong, Qianqian, Xu, Liguang, Wang, Libing, Xu, Chuanlai
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biosensing Techniques - instrumentation Biotechnology Detection DNA DNA - analysis DNA - genetics Equipment Design Equipment Failure Analysis fluorescence Fundamental and applied biological sciences. Psychology genes gold Gold - chemistry Interface PCR Nanoparticles Nanoparticles - chemistry polymerase chain reaction Polymerase Chain Reaction - instrumentation Quantum Dots rapid methods Sequence Analysis, DNA - instrumentation Spectrometry, Fluorescence - instrumentation
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Beschreibung
Zusammenfassung:	A novel, rapid DNA detection method based on fluorescence quenching of quantum dots (QDs) by gold nanoparticles (GNPs) through polymerase chain reaction (PCR) was developed. In proof-of-concept experiments, the length of the amplicon DNA ranging from 152 to 1003 base pairs (bp) could be determined based on quenched fluorescence intensity with 136 bp as the lower limit of effective range. And the real sample detections were also achieved successfully by this developed method. Therefore, this DNA detection method has the potential to be the powerful gene diagnostic tool.
ISSN:	0956-5663 1873-4235
DOI:	10.1016/j.bios.2010.10.043