The effects of luteolin on phenoloxidase and the growth of Spodoptera exigua (Hübner) larvae (Lepidoptera: Noctuidae)
The effects of luteolin on phenoloxidase (PO) from Spodoptera exigua larvae were investigated with the use of a microtitration assay in the present paper. The results showed that luteolin could inhibit the activity of PO and the concentration of this inhibitor that led to a 50% (IC50) activity reduc...
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Veröffentlicht in: | Journal of Pesticide Science 2010/11/25, Vol.35(4), pp.483-487 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The effects of luteolin on phenoloxidase (PO) from Spodoptera exigua larvae were investigated with the use of a microtitration assay in the present paper. The results showed that luteolin could inhibit the activity of PO and the concentration of this inhibitor that led to a 50% (IC50) activity reduction was estimated to be 0.47 mmol/l. The inhibitory kinetics were analyzed by Lineweaver–Burk plots and the compound was found to be a reversible competitive inhibitor with a Ki of 13.11 mmol/l. The compound was obviously toxic against the larvae (≤3rd instar) of Spodoptera exigua. The highest inhibitory percentage in the older larvae (≥3rd instar) was greater than 60% with luteolin treatment. The pupation rate and percentage of eclosion was also evidently reduced with luteolin treatment by the ingestion method in this investigation. |
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ISSN: | 1348-589X 1349-0923 1349-0923 |
DOI: | 10.1584/jpestics.G10-24 |