Photophysical Characterization of a FRET System Using Tailor-Made DNA Oligonucleotide Sequences
We have carried out a detailed photophysical study of the FRET D/A pair consisting of a carbostyril donor and a Ru(II)bathophenanthroline complex acceptor in double-stranded synthetic DNA. Altogether 13 different double-stranded 30 base pair DNAs showing small incremental differences in the distance...
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| Veröffentlicht in: | Bioconjugate chemistry 2010-12, Vol.21 (12), p.2347-2354 |
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| container_issue | 12 |
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| container_title | Bioconjugate chemistry |
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| creator | Flehr, Roman Kienzler, Andrea Bannwarth, Willi Kumke, Michael U. |
| description | We have carried out a detailed photophysical study of the FRET D/A pair consisting of a carbostyril donor and a Ru(II)bathophenanthroline complex acceptor in double-stranded synthetic DNA. Altogether 13 different double-stranded 30 base pair DNAs showing small incremental differences in the distances between donor and acceptor were synthesized. Using the fluorescence of the donor as well as of the acceptor, D/A separations were determined and compared to those derived from a well-established model for DNA distance calculations. The model calculations and anisotropy studies revealed that the donor can nearly be seen as a free rotator allowing the application of the established FRET data evaluation. |
| doi_str_mv | 10.1021/bc100389k |
| format | Article |
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Altogether 13 different double-stranded 30 base pair DNAs showing small incremental differences in the distances between donor and acceptor were synthesized. Using the fluorescence of the donor as well as of the acceptor, D/A separations were determined and compared to those derived from a well-established model for DNA distance calculations. The model calculations and anisotropy studies revealed that the donor can nearly be seen as a free rotator allowing the application of the established FRET data evaluation.</description><identifier>ISSN: 1043-1802</identifier><identifier>EISSN: 1520-4812</identifier><identifier>DOI: 10.1021/bc100389k</identifier><identifier>PMID: 21114283</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Anisotropy ; Base Sequence ; Biochemistry ; Deoxyribonucleic acid ; DNA ; DNA - chemical synthesis ; DNA - metabolism ; Fluorescence ; Fluorescence Resonance Energy Transfer - methods ; Fluorescent Dyes ; Hydroxyquinolines - metabolism ; Models, Molecular ; Oligonucleotides - chemical synthesis ; Oligonucleotides - metabolism ; Organometallic Compounds - metabolism ; Phenanthrolines - metabolism ; Photoelectron Spectroscopy ; Polymers ; Quinolones - metabolism ; Spectrometry, Fluorescence</subject><ispartof>Bioconjugate chemistry, 2010-12, Vol.21 (12), p.2347-2354</ispartof><rights>Copyright © 2010 American Chemical Society</rights><rights>Copyright American Chemical Society Dec 15, 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a407t-f35760364548bfc6ea312196be872f8da385818fd99fa03489fd705895ff404f3</citedby><cites>FETCH-LOGICAL-a407t-f35760364548bfc6ea312196be872f8da385818fd99fa03489fd705895ff404f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bc100389k$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bc100389k$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21114283$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Flehr, Roman</creatorcontrib><creatorcontrib>Kienzler, Andrea</creatorcontrib><creatorcontrib>Bannwarth, Willi</creatorcontrib><creatorcontrib>Kumke, Michael U.</creatorcontrib><title>Photophysical Characterization of a FRET System Using Tailor-Made DNA Oligonucleotide Sequences</title><title>Bioconjugate chemistry</title><addtitle>Bioconjugate Chem</addtitle><description>We have carried out a detailed photophysical study of the FRET D/A pair consisting of a carbostyril donor and a Ru(II)bathophenanthroline complex acceptor in double-stranded synthetic DNA. Altogether 13 different double-stranded 30 base pair DNAs showing small incremental differences in the distances between donor and acceptor were synthesized. Using the fluorescence of the donor as well as of the acceptor, D/A separations were determined and compared to those derived from a well-established model for DNA distance calculations. The model calculations and anisotropy studies revealed that the donor can nearly be seen as a free rotator allowing the application of the established FRET data evaluation.</description><subject>Anisotropy</subject><subject>Base Sequence</subject><subject>Biochemistry</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - chemical synthesis</subject><subject>DNA - metabolism</subject><subject>Fluorescence</subject><subject>Fluorescence Resonance Energy Transfer - methods</subject><subject>Fluorescent Dyes</subject><subject>Hydroxyquinolines - metabolism</subject><subject>Models, Molecular</subject><subject>Oligonucleotides - chemical synthesis</subject><subject>Oligonucleotides - metabolism</subject><subject>Organometallic Compounds - metabolism</subject><subject>Phenanthrolines - metabolism</subject><subject>Photoelectron Spectroscopy</subject><subject>Polymers</subject><subject>Quinolones - metabolism</subject><subject>Spectrometry, Fluorescence</subject><issn>1043-1802</issn><issn>1520-4812</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkFtLxDAQhYMo3h_8AxIEER-qk0vb5FHWK6gruj6XbJq40W6zJu3D-uuN7KqgMDDD8HHO4SC0R-CEACWnY00AmJBvK2iT5BQyLghdTTdwlhEBdANtxfgKAJIIuo42KCGEU8E2UfUw8Z2fTebRadXgwUQFpTsT3IfqnG-xt1jhy8eLEX6ax85M8XN07QseKdf4kN2p2uDz-zM8bNyLb3vdGN-59Hsy771ptYk7aM2qJprd5d5Gz5cXo8F1dju8uhmc3WaKQ9llluVlAazgORdjqwujGKFEFmMjSmpFrZjIBRG2ltIqYFxIW5eQC5lby4Fbto2OFrqz4JN17Kqpi9o0jWqN72MlClIKyRgk8uAP-er70KZwlUiWkqdJ0PEC0sHHGIytZsFNVZhXBKqvzqufzhO7vxTsx1NT_5DfJSfgcAEoHX_N_gt9AhYJhlI</recordid><startdate>20101215</startdate><enddate>20101215</enddate><creator>Flehr, Roman</creator><creator>Kienzler, Andrea</creator><creator>Bannwarth, Willi</creator><creator>Kumke, Michael U.</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20101215</creationdate><title>Photophysical Characterization of a FRET System Using Tailor-Made DNA Oligonucleotide Sequences</title><author>Flehr, Roman ; 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| ispartof | Bioconjugate chemistry, 2010-12, Vol.21 (12), p.2347-2354 |
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| source | ACS Publications; MEDLINE |
| subjects | Anisotropy Base Sequence Biochemistry Deoxyribonucleic acid DNA DNA - chemical synthesis DNA - metabolism Fluorescence Fluorescence Resonance Energy Transfer - methods Fluorescent Dyes Hydroxyquinolines - metabolism Models, Molecular Oligonucleotides - chemical synthesis Oligonucleotides - metabolism Organometallic Compounds - metabolism Phenanthrolines - metabolism Photoelectron Spectroscopy Polymers Quinolones - metabolism Spectrometry, Fluorescence |
| title | Photophysical Characterization of a FRET System Using Tailor-Made DNA Oligonucleotide Sequences |
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