Photophysical Characterization of a FRET System Using Tailor-Made DNA Oligonucleotide Sequences

We have carried out a detailed photophysical study of the FRET D/A pair consisting of a carbostyril donor and a Ru(II)bathophenanthroline complex acceptor in double-stranded synthetic DNA. Altogether 13 different double-stranded 30 base pair DNAs showing small incremental differences in the distance...

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Veröffentlicht in:Bioconjugate chemistry 2010-12, Vol.21 (12), p.2347-2354
Hauptverfasser: Flehr, Roman, Kienzler, Andrea, Bannwarth, Willi, Kumke, Michael U.
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Sprache:eng
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Zusammenfassung:We have carried out a detailed photophysical study of the FRET D/A pair consisting of a carbostyril donor and a Ru(II)bathophenanthroline complex acceptor in double-stranded synthetic DNA. Altogether 13 different double-stranded 30 base pair DNAs showing small incremental differences in the distances between donor and acceptor were synthesized. Using the fluorescence of the donor as well as of the acceptor, D/A separations were determined and compared to those derived from a well-established model for DNA distance calculations. The model calculations and anisotropy studies revealed that the donor can nearly be seen as a free rotator allowing the application of the established FRET data evaluation.
ISSN:1043-1802
1520-4812
DOI:10.1021/bc100389k