Photophysical Characterization of a FRET System Using Tailor-Made DNA Oligonucleotide Sequences
We have carried out a detailed photophysical study of the FRET D/A pair consisting of a carbostyril donor and a Ru(II)bathophenanthroline complex acceptor in double-stranded synthetic DNA. Altogether 13 different double-stranded 30 base pair DNAs showing small incremental differences in the distance...
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Veröffentlicht in: | Bioconjugate chemistry 2010-12, Vol.21 (12), p.2347-2354 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | We have carried out a detailed photophysical study of the FRET D/A pair consisting of a carbostyril donor and a Ru(II)bathophenanthroline complex acceptor in double-stranded synthetic DNA. Altogether 13 different double-stranded 30 base pair DNAs showing small incremental differences in the distances between donor and acceptor were synthesized. Using the fluorescence of the donor as well as of the acceptor, D/A separations were determined and compared to those derived from a well-established model for DNA distance calculations. The model calculations and anisotropy studies revealed that the donor can nearly be seen as a free rotator allowing the application of the established FRET data evaluation. |
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ISSN: | 1043-1802 1520-4812 |
DOI: | 10.1021/bc100389k |