Barsta:barnase -- a versatile platform for colloidal diamond bioconjugation

Barsta:barnase -- a versatile platform for colloidal diamond bioconjugation

We report on a bioconjugation platform based on a high-affinity protein pair, barstar:barnase (Bs:Bn), that provides a modular design toolkit capable of strong (covalent) and versatile assembly of bio/nanocomplexes. Luminescent nanodiamonds (140 nm) serve as the docking station to synthesize Bs:Bn b...

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Veröffentlicht in:Journal of materials chemistry 2011-01, Vol.21 (1), p.65-68
Hauptverfasser: Sreenivasan, VKA, Ivukina, E A, Deng, W, Keif, T A, Zdobnova, T A, Lukash, S V, Veryugin, B V, Stremovskiy, O A, Zvyagin, A V, Deyev, S M
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Sprache:eng
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Zusammenfassung:We report on a bioconjugation platform based on a high-affinity protein pair, barstar:barnase (Bs:Bn), that provides a modular design toolkit capable of strong (covalent) and versatile assembly of bio/nanocomplexes. Luminescent nanodiamonds (140 nm) serve as the docking station to synthesize Bs:Bn bioconjugates which are characterized and utilized in several applications, including cell transfection.
ISSN:0959-9428
DOI:10.1039/c0jm02819c