Enhancement of sortase A-mediated protein ligation by inducing a β-hairpin structure around the ligation site

A Staphylococcus aureus transpeptidase, sortase A (SrtA), catalyzes selective peptide/protein ligations that have been applied to cell imaging and protein engineering, while the ligations do not proceed to completion due to their reversibility. We successfully enhanced SrtA-mediated protein ligation...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2011-04, Vol.47 (16), p.4742-4744
Hauptverfasser:	Yamamura, Yuichi, Hirakawa, Hidehiko, Yamaguchi, Satoshi, Nagamune, Teruyuki
Format:	Artikel
Sprache:	eng
Schlagworte:	Aminoacyltransferases - chemistry Aminoacyltransferases - metabolism Bacterial Proteins - chemistry Bacterial Proteins - metabolism Binding Sites Biocatalysis Cysteine Endopeptidases - chemistry Cysteine Endopeptidases - metabolism Models, Molecular Protein Engineering Protein Structure, Secondary Proteins - chemical synthesis Proteins - chemistry Staphylococcus aureus - enzymology Temperature
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A Staphylococcus aureus transpeptidase, sortase A (SrtA), catalyzes selective peptide/protein ligations that have been applied to cell imaging and protein engineering, while the ligations do not proceed to completion due to their reversibility. We successfully enhanced SrtA-mediated protein ligation through the formation of a β-hairpin around the ligation site.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c0cc05334a