Performance of chloroperoxidase stabilization in mesoporous sol-gel glass using in situ glucose oxidase peroxide generation
A unique mesoporous sol-gel glass possessing a highly ordered porous structure (with three pore sizes of about 50, 150, and 200 A diameter) was used as a support material for immobilization of the enzyme chloroperoxidase (CPO). CPO was bound onto the glass via a bifunctional ligand, trimethoxysilylp...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2004, Vol.113-116 (1-3), p.273-285 |
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| container_title | Applied biochemistry and biotechnology |
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| creator | Borole, Abhijeet Dai, Sheng Cheng, Catherine L Rodriguez, Jr, Miguel Davison, Brian H |
| description | A unique mesoporous sol-gel glass possessing a highly ordered porous structure (with three pore sizes of about 50, 150, and 200 A diameter) was used as a support material for immobilization of the enzyme chloroperoxidase (CPO). CPO was bound onto the glass via a bifunctional ligand, trimethoxysilylpropanal. In situ production of the cosubstrate, H2O2, was achieved using glucose oxidase. Solvent stability in acetonitrile mixtures was enhanced when a pore size larger than the size of CPO was used (i.e., 200 A). From these results, it appears that the glass-enzyme complex developed through the present work can be used as high-performance biocatalysts for various chemical-processing applications, particularly in harsh conditions. |
| doi_str_mv | 10.1385/ABAB:113:1-3:273 |
| format | Article |
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| ispartof | Applied biochemistry and biotechnology, 2004, Vol.113-116 (1-3), p.273-285 |
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| subjects | Acetonitrile Aldehydes - chemistry Biocatalysts Biosensors Biotechnology - methods Catalysis Chloride Peroxidase - chemistry Enzymes Glass Glucose oxidase Glucose Oxidase - chemistry Horseradish Peroxidase - chemistry Hydrogen peroxide Hydrogen Peroxide - chemistry Immobilization Ligands Models, Chemical Peroxides - chemistry Phase Transition Pore size Silanes - chemistry Sol-gel processes Solvents Studies Temperature |
| title | Performance of chloroperoxidase stabilization in mesoporous sol-gel glass using in situ glucose oxidase peroxide generation |
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