Transformation of dendritic cells from plasmacytoid to myeloid in a leukemic plasmacytoid dendritic cell line (PMDC05)

Abstract We investigated the transformation from pDCs to mDCs in a pDC line (PMDC05) which was established from a patient with pDC leukemia in our laboratory. PMDC05 cells were separated into two fractions according to the expression of BDCA1 and CD123. BDCA1− CD123+ cells were found to be pDC-like...

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Veröffentlicht in:	Leukemia research 2010-11, Vol.34 (11), p.1517-1524
Hauptverfasser:	Watanabe, Norihiro, Narita, Miwako, Yamahira, Akie, Nakamura, Takeshi, Tochiki, Nozomi, Saitoh, Anri, Kaji, Masami, Hashimoto, Shigeo, Furukawa, Tatsuo, Toba, Ken, Fuse, Ichiro, Aizawa, Yoshifusa, Takahashi, Masuhiro
Format:	Artikel
Sprache:	eng
Schlagworte:	CD123 antigen Cell Line, Tumor Cell Lineage Cell Transformation, Neoplastic Cells, Cultured Dendritic Cells - pathology Hematology, Oncology and Palliative Medicine Humans Leukemia - pathology Leukemic plasmacytoid dendritic cell line Myeloid dendritic cell Plasmacytoid dendritic cell Transformation
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Zusammenfassung:	Abstract We investigated the transformation from pDCs to mDCs in a pDC line (PMDC05) which was established from a patient with pDC leukemia in our laboratory. PMDC05 cells were separated into two fractions according to the expression of BDCA1 and CD123. BDCA1− CD123+ cells were found to be pDC-like cells by their morphology, surface phenotypes, mRNA expression and the function. In addition, BDCA1− CD123+ cells were demonstrated to have a proliferating capacity and revealed the ability to transform to BDCA1+ CD123− cells which showed mDC-like properties. Our data demonstrated the possibility of transformation from pDCs to mDCs in human DC lineage.
ISSN:	0145-2126 1873-5835
DOI:	10.1016/j.leukres.2010.03.002