Altered Expression of Cell Cycle and Apoptotic Proteins in Human Liver Pathologies
Τhe expression of cell cycle (P53, Ki-67, P21, and P27) and apoptotic proteins (BCL-2 and BAX) was investigated by immunohistochemistry in paraffin-embedded formalin-fixed tissues of normal and pathologic liver. An increased frequency of expression of P21 in cirrhosis and hepatocellular carcinoma (H...
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| Veröffentlicht in: | Anticancer research 2010-11, Vol.30 (11), p.4493-4501 |
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| creator | MITSELOU, Antigony KARAPIPERIDES, Damianos NESSERIS, Ioannis VOUGIOUKLAKIS, Theodoros AGNANTIS, Niki J |
| description | Τhe expression of cell cycle (P53, Ki-67, P21, and P27) and apoptotic proteins (BCL-2 and BAX) was investigated by immunohistochemistry in paraffin-embedded formalin-fixed tissues of normal and pathologic liver. An increased frequency of expression of P21 in cirrhosis and hepatocellular carcinoma (HCC) (p=0.003 and p=0.001 respectively) was found; P27 protein expression was more frequent in hepatitis (p=0.001) and HCC (p=0.003) when compared with normal tissue. BCL-2 protein was markedly more frequent in steatohepatitis (p |
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An increased frequency of expression of P21 in cirrhosis and hepatocellular carcinoma (HCC) (p=0.003 and p=0.001 respectively) was found; P27 protein expression was more frequent in hepatitis (p=0.001) and HCC (p=0.003) when compared with normal tissue. BCL-2 protein was markedly more frequent in steatohepatitis (p<0.05) as compared to normal liver, in hepatitis cases (p=0.002) and in metastases (p<0.033). The expression of BAX was more frequent in hepatitis (p=0.001) and cirrhosis (p<0.001). We demonstrated in our study the expression of these proteins at different levels in liver pathologies. These findings have implications for understanding the evolution from liver inflammation to cirrhosis and associated carcinogenesis.</description><identifier>ISSN: 0250-7005</identifier><identifier>EISSN: 1791-7530</identifier><identifier>PMID: 21115898</identifier><language>eng</language><publisher>Attiki: International Institute of Anticancer Research</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Apoptosis ; bcl-2-Associated X Protein - metabolism ; Biological and medical sciences ; Carcinoma, Hepatocellular - metabolism ; Carcinoma, Hepatocellular - pathology ; Cell Cycle Proteins - metabolism ; Cyclin-Dependent Kinase Inhibitor p21 - metabolism ; Fatty Liver - metabolism ; Fatty Liver - pathology ; Female ; Gastroenterology. Liver. Pancreas. Abdomen ; Humans ; Immunoenzyme Techniques ; Ki-67 Antigen - metabolism ; Liver - metabolism ; Liver - pathology ; Liver Cirrhosis - metabolism ; Liver Cirrhosis - pathology ; Liver Neoplasms - metabolism ; Liver Neoplasms - pathology ; Liver. Biliary tract. Portal circulation. Exocrine pancreas ; Male ; Medical sciences ; Middle Aged ; Other diseases. Semiology ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Tumor Suppressor Protein p53 - metabolism ; Tumors</subject><ispartof>Anticancer research, 2010-11, Vol.30 (11), p.4493-4501</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23530748$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21115898$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MITSELOU, Antigony</creatorcontrib><creatorcontrib>KARAPIPERIDES, Damianos</creatorcontrib><creatorcontrib>NESSERIS, Ioannis</creatorcontrib><creatorcontrib>VOUGIOUKLAKIS, Theodoros</creatorcontrib><creatorcontrib>AGNANTIS, Niki J</creatorcontrib><title>Altered Expression of Cell Cycle and Apoptotic Proteins in Human Liver Pathologies</title><title>Anticancer research</title><addtitle>Anticancer Res</addtitle><description>Τhe expression of cell cycle (P53, Ki-67, P21, and P27) and apoptotic proteins (BCL-2 and BAX) was investigated by immunohistochemistry in paraffin-embedded formalin-fixed tissues of normal and pathologic liver. An increased frequency of expression of P21 in cirrhosis and hepatocellular carcinoma (HCC) (p=0.003 and p=0.001 respectively) was found; P27 protein expression was more frequent in hepatitis (p=0.001) and HCC (p=0.003) when compared with normal tissue. BCL-2 protein was markedly more frequent in steatohepatitis (p<0.05) as compared to normal liver, in hepatitis cases (p=0.002) and in metastases (p<0.033). The expression of BAX was more frequent in hepatitis (p=0.001) and cirrhosis (p<0.001). We demonstrated in our study the expression of these proteins at different levels in liver pathologies. These findings have implications for understanding the evolution from liver inflammation to cirrhosis and associated carcinogenesis.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Apoptosis</subject><subject>bcl-2-Associated X Protein - metabolism</subject><subject>Biological and medical sciences</subject><subject>Carcinoma, Hepatocellular - metabolism</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>Cyclin-Dependent Kinase Inhibitor p21 - metabolism</subject><subject>Fatty Liver - metabolism</subject><subject>Fatty Liver - pathology</subject><subject>Female</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Ki-67 Antigen - metabolism</subject><subject>Liver - metabolism</subject><subject>Liver - pathology</subject><subject>Liver Cirrhosis - metabolism</subject><subject>Liver Cirrhosis - pathology</subject><subject>Liver Neoplasms - metabolism</subject><subject>Liver Neoplasms - pathology</subject><subject>Liver. Biliary tract. Portal circulation. Exocrine pancreas</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Other diseases. Semiology</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Tumor Suppressor Protein p53 - metabolism</subject><subject>Tumors</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E1LAzEQgOEgiq3VvyC5iKeFfDQfeyxLtULBIr0vyWaqkexmTXbF_nsXrHj0NJeHYeY9Q3OqSloowck5mhMmSKEIETN0lfM7IVKWml-iGaOUCl3qOXpZhQESOLz-6hPk7GOH4wFXEAKujk0AbDqHV33shzj4Bu9SHMB3GfsOb8bWdHjrPyHhnRneYoivHvI1ujiYkOHmNBdo_7DeV5ti-_z4VK22Rc8UGwpLlXRMUWeoKLXjVBC1XEphGIPpCW6BWmW1phKEcFwRobRVklgnKSOOL9D9z9o-xY8R8lC3PjfT3aaDOOZaS8LVFED8L6kQk1NykrcnOdoWXN0n35p0rH97TeDuBExuTDgk0zU-_zk-hVdLzb8BcvxydA</recordid><startdate>20101101</startdate><enddate>20101101</enddate><creator>MITSELOU, Antigony</creator><creator>KARAPIPERIDES, Damianos</creator><creator>NESSERIS, Ioannis</creator><creator>VOUGIOUKLAKIS, Theodoros</creator><creator>AGNANTIS, Niki J</creator><general>International Institute of Anticancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20101101</creationdate><title>Altered Expression of Cell Cycle and Apoptotic Proteins in Human Liver Pathologies</title><author>MITSELOU, Antigony ; KARAPIPERIDES, Damianos ; NESSERIS, Ioannis ; VOUGIOUKLAKIS, Theodoros ; AGNANTIS, Niki J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p272t-b176d271da1598d315074465a22e1793be1b7b8816e55d370578b760bd6120d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Apoptosis</topic><topic>bcl-2-Associated X Protein - metabolism</topic><topic>Biological and medical sciences</topic><topic>Carcinoma, Hepatocellular - metabolism</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Cell Cycle Proteins - metabolism</topic><topic>Cyclin-Dependent Kinase Inhibitor p21 - metabolism</topic><topic>Fatty Liver - metabolism</topic><topic>Fatty Liver - pathology</topic><topic>Female</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Ki-67 Antigen - metabolism</topic><topic>Liver - metabolism</topic><topic>Liver - pathology</topic><topic>Liver Cirrhosis - metabolism</topic><topic>Liver Cirrhosis - pathology</topic><topic>Liver Neoplasms - metabolism</topic><topic>Liver Neoplasms - pathology</topic><topic>Liver. Biliary tract. Portal circulation. Exocrine pancreas</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Other diseases. Semiology</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Tumor Suppressor Protein p53 - metabolism</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MITSELOU, Antigony</creatorcontrib><creatorcontrib>KARAPIPERIDES, Damianos</creatorcontrib><creatorcontrib>NESSERIS, Ioannis</creatorcontrib><creatorcontrib>VOUGIOUKLAKIS, Theodoros</creatorcontrib><creatorcontrib>AGNANTIS, Niki J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MITSELOU, Antigony</au><au>KARAPIPERIDES, Damianos</au><au>NESSERIS, Ioannis</au><au>VOUGIOUKLAKIS, Theodoros</au><au>AGNANTIS, Niki J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Altered Expression of Cell Cycle and Apoptotic Proteins in Human Liver Pathologies</atitle><jtitle>Anticancer research</jtitle><addtitle>Anticancer Res</addtitle><date>2010-11-01</date><risdate>2010</risdate><volume>30</volume><issue>11</issue><spage>4493</spage><epage>4501</epage><pages>4493-4501</pages><issn>0250-7005</issn><eissn>1791-7530</eissn><abstract>Τhe expression of cell cycle (P53, Ki-67, P21, and P27) and apoptotic proteins (BCL-2 and BAX) was investigated by immunohistochemistry in paraffin-embedded formalin-fixed tissues of normal and pathologic liver. An increased frequency of expression of P21 in cirrhosis and hepatocellular carcinoma (HCC) (p=0.003 and p=0.001 respectively) was found; P27 protein expression was more frequent in hepatitis (p=0.001) and HCC (p=0.003) when compared with normal tissue. BCL-2 protein was markedly more frequent in steatohepatitis (p<0.05) as compared to normal liver, in hepatitis cases (p=0.002) and in metastases (p<0.033). The expression of BAX was more frequent in hepatitis (p=0.001) and cirrhosis (p<0.001). We demonstrated in our study the expression of these proteins at different levels in liver pathologies. These findings have implications for understanding the evolution from liver inflammation to cirrhosis and associated carcinogenesis.</abstract><cop>Attiki</cop><pub>International Institute of Anticancer Research</pub><pmid>21115898</pmid><tpages>9</tpages></addata></record> |
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| subjects | Adult Aged Aged, 80 and over Apoptosis bcl-2-Associated X Protein - metabolism Biological and medical sciences Carcinoma, Hepatocellular - metabolism Carcinoma, Hepatocellular - pathology Cell Cycle Proteins - metabolism Cyclin-Dependent Kinase Inhibitor p21 - metabolism Fatty Liver - metabolism Fatty Liver - pathology Female Gastroenterology. Liver. Pancreas. Abdomen Humans Immunoenzyme Techniques Ki-67 Antigen - metabolism Liver - metabolism Liver - pathology Liver Cirrhosis - metabolism Liver Cirrhosis - pathology Liver Neoplasms - metabolism Liver Neoplasms - pathology Liver. Biliary tract. Portal circulation. Exocrine pancreas Male Medical sciences Middle Aged Other diseases. Semiology Proto-Oncogene Proteins c-bcl-2 - metabolism Tumor Suppressor Protein p53 - metabolism Tumors |
| title | Altered Expression of Cell Cycle and Apoptotic Proteins in Human Liver Pathologies |
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