A specific and sensitive method for detection of hypochlorous acid for the imaging of microbe-induced HOCl production

A specific and sensitive fluorescence-based method was developed for the imaging of microbe-induced HOCl production. Furthermore, we demonstrate dual oxidase (DUOX)-mediated HOCl generation in the mucosa of live animals providing a novel insight into mucosal innate immunity.

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Bibliographische Detailangaben
Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2011-01, Vol.47 (15), p.4373-4375
Hauptverfasser:	Chen, Xiaoqiang, Lee, Kyung-Ah, Ha, Eun-Mi, Lee, Kyung Mi, Seo, You Yeong, Choi, Han Kyoung, Kim, Ha Na, Kim, Min Jung, Cho, Chun-Seok, Lee, Soo Young, Lee, Won-Jae, Yoon, Juyoung
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Physiological Phenomena Cell Survival Drosophila melanogaster - immunology Drosophila melanogaster - microbiology Fungi - physiology Humans Hypochlorous Acid - metabolism Immunity, Innate Intestinal Mucosa - metabolism Intestinal Mucosa - microbiology Molecular Imaging - methods NADPH Oxidases - metabolism Neutrophils - cytology Neutrophils - microbiology Phagosomes - metabolism Rhodamines - chemistry
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Beschreibung
Zusammenfassung:	A specific and sensitive fluorescence-based method was developed for the imaging of microbe-induced HOCl production. Furthermore, we demonstrate dual oxidase (DUOX)-mediated HOCl generation in the mucosa of live animals providing a novel insight into mucosal innate immunity.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c1cc10589b